CXCR4和CD133mRNA在胃腺癌原发灶中的表达及其临床意义  

Expressions and significances of CXCR4 and CD133mRNA in primary lesion of gastric adenocarcinoma

作　　者：周国才[1] 俞继卫[1] 陆瑞祺[1] 吴巨钢[1] 姜海广[1] 倪晓春[1] 郑林海[1] 姜波健[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院普外科,上海201900

出　　处：《国际外科学杂志》2012年第3期166-170,F0003,共6页International Journal of Surgery

基　　金：国家自然基金资助项目（No.81101850）;上海市科委引领课题（No.09411962300）;上海交通大学医学院课题（No.YZ1055）

摘　　要：目的 探讨CXCR4和CDl33mRNA在胃腺癌原发灶中的表达及其与各临床病理参数之间的关系。分析CXCR4和CDl33mRNA二者间的相关关系。方法 分析50例胃腺癌原发灶和癌旁胃黏膜组织行免疫组化法定位检测CXCR4和CD133mRNA的相对表达率，并用半定量RT-PCR法测定CXCR4和CDl33mRNA的相对灰度值，分析上述两者间的相互关系（Fisher精确概率法）。分析CXCR4和CDl33mRNA与临床病理因素之间的联系（Spearman相关分析）。结果 CXCR4和CDl33mRNA在胃腺原发灶中的阳性表达率分别为76．0％和66．0％，显著高于癌旁胃黏膜组织中的16．0％（P=0．000）和10．0％（P=0．000）。肿瘤直径越大、TNM分期越晚及淋巴管浸润存在，则CXCR4mRNA灰度值就越高（P〈0．05）。肿瘤较大，TNM分期较晚者，CDl33mRNA灰度值越高（P〈0．05）。CXCR4和CDl33mRNA相对灰度值变化呈正相关（r=0．453，P〈0．01）。结论 CXCR4和CDl33mRNA的高表达与胃癌的肿瘤直径、TNM分期及淋巴管浸润有关。CD133mRNA相对灰度值随CXCR4mRNA相对灰摩信增加而增加．Objective To investigate the expression of CXCR4 and CD133mRNA in primary lesion of primary gastric ad and the relation with clinicopathological features, and to explore the correlation of CXCR4 and CD133. Methods The primary lesion of primary gastric adenocarcinoma and normal tissues adjacent to gastric cancer were obtained from 50 patients. The diction of CXCR4 and CD133 protein expression was detected by the immunohistochemical staining, and the relative gray scale values of CXCR4 and CD133mRNA by semi-quantitative RT-PCR （Fisher＇ s exact probability method）. Their relationship with clinicopathological features was also investigated （Spearman relation analysis）. Results The positive rates of CXCR4 and CD133 protein in gastric cancers were 76.0% and 66.0% respectively, which were significantly higher than that in normal tissues adjacent to gastric cancer （ 16.0% and 10% ; P = 0. 000, P = 0.000）. The increment of relative gray scale values of CXCR4mRNA was associated with the larger tumor diameter, the later TNM stage and the occurrence of lymphatic metastasis（ P 〈 0.05 ）. And the larger diame- ter of tumor, the later TNM stage were associated with the higher relative gray scale values of CD133mRNA （P 〈 0.05 ）. The levels of the relative gray scale values of CXCR4 mRNA and CD133mRNA were positively related（ r = 0.453 ,P 〈0.01 ）. Conclusions The higher expression of CXCR4 and CD133mRNA correlates with tumour diameter, TNM stage and lymphatic vessel invasion. The relative gray scale values of CD133mRNA increase with the increment of the relative gray scale values of CXCR4.

关 键 词：胃 新生物/癌 干细胞 趋化因子 CXC 胃 新生物/癌 干细胞 趋化因子 CXC 

分 类 号：R735.2[医药卫生—肿瘤]