人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4和内向整流性钾离子通道4.1的再分布  

Loss of Perivascular Aquaporin 4 and Inwardly Rectifying Potassium Channel 4.1 in Human Mesial Temporal Lobe Epilepsy

作　　者：徐仟[1,2] 孙振荣[3] 李桂林[1,2] 孙异临[1,2] 杨少华[1,2,3] 袁芳[1,2] 

机构地区：[1]首都医科大学,北京市100069 [2]北京市神经外科研究所,北京市100050 [3]首都医科大学附属北京天坛医院神经外科,北京市100050

出　　处：《中国康复理论与实践》2012年第3期215-218,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金资助项目(30840083)

摘　　要：目的研究人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4(AQP4)和内向整流性钾离子通道4.1(Kir4.1)的分布。方法对10例颞叶内侧癫痫(MTLE)和6例非颞叶内侧癫痫(non-MTLE)手术切除海马组织,应用光镜及透射电镜观察组织学及超微结构,免疫荧光组织化学法观察星形胶质细胞AQP4和Kir4.1的分布。结果 MTLE海马组织星形胶质细胞大量增生伴明显肿胀,神经元显著固缩。non-MTLE海马组织中,AQP4和Kir4.1在星形胶质细胞血管周围足突(pAST-ef)分布多于其他部位,呈现极性分布特点;而在MTLE海马组织中,AQP4和Kir4.1在pAST-ef分布减少,其他部位分布增加,极性分布改变。结论海马组织星形胶质细胞上AQP4和Kir4.1极性分布变化可能与颞叶内侧癫痫发生相关。Objective To investigate the distribution of aquaporin 4（AQP4） and inwardly rectifying potassium channel 4.1（Kir4.1） in the astrocytes from human mesial temporal lobe epilepsy（MTLE）.Methods Hippocampal specimens,including 10 cases of MTLE and 6 cases of non-MTLE,were observed under optical and transmission electron microscopy.The distribution of AQP4 and Kir4.1 in astrocytes was investigated with immunoflurescence.Results Compared with non-MTLE hippocampus,the main structural changes of MTLE included remarkable hyperplasia astrocytes,serious swelling astrocytes and distinguished astrophy neurons.In non-MTLE hippocampus,immunoflurescence signals of AQP4 and Kir4.1 were enriched along perivascular astrocyte end-feet domain.However,it reveals significant loss of AQP4 and Kir4.1 in perivascular astrocyte end-feet domain in MTLE.Conclusion Loss of perivascular AQP4 and Kir4.1 in the human MTLE may help to understand the roles of astrocyte in MTLE.

关 键 词：颞叶内侧癫痫 水通道蛋白4 内向整流性钾离子通道4.1 

分 类 号：R742.1[医药卫生—神经病学与精神病学]