早期干预及康复训练对宫内感染脑损伤仔鼠脑白质髓鞘相关糖蛋白表达的影响  

Effect of Early Intervention and Rehabilitation on Myelin-associated Glycoprotein Expression of White Matter in Neonatal Rats with Cerebral Injury after Intrauterine Infection

作　　者：张明达[1,2,3] 李晓捷[1,2,3] 

机构地区：[1]佳木斯大学附属第三医院脑瘫一科 [2]佳木斯大学儿童神经康复实验室 [3]佳木斯大学康复医学院,黑龙江佳木斯市154003

出　　处：《中国康复理论与实践》2012年第3期229-232,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：2011年佳木斯大学研究生创新科研项目(编号YJSCX2011-017JD)

摘　　要：目的探讨早期干预及康复训练对宫内感染致脑损伤仔鼠脑白质髓鞘相关糖蛋白(MAG)表达的影响。方法 30只孕17 d、18 d的Wistar大鼠腹腔注射脂多糖,抽取所生仔鼠100只随机分干预组和非干预组各50只。另10只孕鼠注射生理盐水,取50只所生仔鼠为对照组。干预组进行早期干预和康复训练,3组分别于14 d、21 d、28 d行改良BBB和悬吊实验评分,各组分别在1 d、7 d、14 d、21 d、28 d随机抽取6只取脑组织行免疫组化染色,观察脑白质MAG的表达情况。结果在悬吊试验和改良BBB测试中,对照组得分均明显高于脂多糖组(P<0.01),干预组得分均明显高于非干预组(P<0.01);脂多糖组脑白质中MAG表达量明显高于对照组(P<0.01),非干预组表达量明显高于干预组(P<0.01)。结论早期干预及康复训练可改善脑损伤鼠的运动功能并能降低MAG的表达。Objective To explore the effect of early intervention on myelin-associated glycoprotein（MAG） expression of white matter in neonatal rats with cerebral brain injury caused by intrauterine infection.Methods 17 days or 18 days pregnant Wistar rats were consecutively injected with lipopolysaccharide（LPS） intraperitoneally.100 neonatal rats born from LPS group were randomly divided into intervention group and non-intervention group with 50 in each group.Another 10 pregnant rats were injected with normal saline,50 neonatal rats born from the the normal saline group were taken as control group.The intervention group received early intervention and rehabilitation training.All the groups underwent hanging test and modified BBB test on the 14th,21st and 28th day.6 rats in each group were tested by immunohistochemical staining to observe the MAG expression in white matter on the 1st,7th,14th,21st,28th day.Results The scores of hanging test and modified BBB test were significantly higher in the control group than in the LPS group（P0.01）,and higher in the intervention group than in the non-intervention group（P0.01）.The expression of MAG was higher in LPS group than in the control group（P0.01）,in non-intervention group than in the intervention group（P0.01）.Conclusion Early intervention and rehabilitation training can improve the motor function in rats with brain injury and reduce the expression of MAG.

关 键 词：早期干预 康复训练 脑损伤 宫内感染 髓鞘相关糖蛋白 

分 类 号：R742.3[医药卫生—神经病学与精神病学]