肺癌放射免疫导向手术的动物实验研究  

The experimental study of radioimmunoguided surgery for lung cancer

作　　者：王钰琦[1] 孙玉鹗[1] 高霭峰 

机构地区：[1]解放军总医院胸外科,北京100853 [2]沈阳铁路总医院病理科

出　　处：《中国肺癌杂志》2000年第1期46-49,共4页Chinese Journal of Lung Cancer

摘　　要：目的　探讨放射免疫导向手术在检测小鼠肺腺癌动物模型肿瘤转移的灵敏度、特异性及可靠性。方法　应用抗人肺腺癌单克隆抗体LC 1生物素化预定位技术 ,进行肺腺癌小鼠放射免疫导向手术。结果　γ照相机体外显像和井型探测仪体内放射性分布探测的结果显示 :在注射放射性核素 2h后 ,生物素化抗体预定位的荷瘤小鼠的肿瘤即有明显放射性浓集 ,其原发肿瘤和肺部转移灶的T/NT比值均显著高于未进行生物素化抗体预定位的荷瘤小鼠 (P <0 .0 1)。应用手持型γ探测仪 (GDP)对荷瘤小鼠进行体内探测 ,判别小鼠肺部肿瘤转移的灵敏度为 96% ,特异性为 98% ,准确率为 97%。结论　采用抗肺腺癌单克隆抗体LC 1生物素化预定位技术能明显提高小鼠肿瘤的放射性浓集 。Objective To determine the sensitivity, specificity and reliability of radioimmunoguided surgery in detecting the tumor micrometastases of the mice models bearing pulmonary adenocarcinoma.Methods Radioimmunoguided surgery was performed on mice models bearing pulmonary adenocarcinoma with antihuman lung adenocarcinoma monoclonal antibody (LC 1) by biotin avidin pretargeting technique.Results The result of γ camera radioimmunoimaging and the data of radioactivity biodistribution provided by standard gamma well counts showed: as early as 2 hours after injection, radioactivity had accumulated in tumors of the biotinylated monoclonal antibodies pretargeting mice. The T/NT ratios of the primary tumors and the metastases in the lung were significantly higher in the biotinylated monoclonal antibodies pretargeting mice than those without undergoing such treatment (P<0.01). Based on the application of GDP to identify the metastases in the lung ,the sensitivity, specificity and accuracy of RIGS were 96%, 98% and 97% respectively. Conclusion Biotin avidin pretargeting techniques could remarkably enhance the radioactive deposition on tumors of the biotinylated LC 1 pretargeting mice. The primary tumors and the micrometastases in the lung could be detected in vivo by GDP.

关 键 词：放射导向手术 BAS 肺肿瘤 荷瘤小鼠 

分 类 号：R734.2[医药卫生—肿瘤]