大鼠脑干出血后血清S100B表达与血肿周围组织的超微结构改变  

Serum S100B expression and the change of perihematoma tissue ultra microstructure protein in brain tissue after brainstem hemorrhage in rats

作　　者：张海涛[1,2] 王永和[2] 曹培成[2] 卜振富[2] 李爱军[2] 程迎新[2] 

机构地区：[1]潍坊医学院,山东潍坊261053 [2]潍坊市人民医院脑科医院神经外科,山东潍坊261021

出　　处：《国际神经病学神经外科学杂志》2012年第1期7-11,共5页Journal of International Neurology and Neurosurgery

摘　　要：目的研究大鼠脑干出血后血清S100B的表达及其与行为学改变、脑水肿的关系。观察血肿周围组织超微结构在脑干水肿形成中的变化。方法利用立体定向技术制作大鼠脑干出血模型,采用双抗体夹心ELISA法检测脑干出血后血清S100B蛋白含量,干湿重法检测脑组织含水量,电镜观察脑干出血后血肿周围组织超微结构的变化。结果与假手术组比较,出血组在脑干出血后6 h血清S100B含量、行为学改变、脑组织含水量增高,48 h达高峰,之后开始下降;脑干出血后血清S100B蛋白含量、脑组织含水量、行为学改变三者之间相互存在关联(P<0.05)。脑干出血后血肿周围组织电镜观察:脑干出血后6 h星形胶质细胞胞体和周边足突轻度肿胀,48 h可见星形胶质细胞肿胀明显且部分胞浆呈空泡样改变;髓鞘部分有剥离现象,并有线粒体嵴断裂及空泡样变。结论脑干出血后血清S100B含量、行为学改变、脑组织含水量三者之间相互呈正相关,提示脑干出血后高水平的S100B蛋白参与脑干出血后脑水肿的形成。Objective To investigate the correlation of serum S100B expression with behavioral tests and cerebral edema . Examine the changes of perihematoma tissue ultra microstructure in the development of brain edema. Methods A rat model of brainstem hem- orrhage was established using the stereotactic technology, serum S100B was detected by double antibody sandwich ELISA , Brain water contents were measured by dry-wet weigh method. Perihematoma tissue ultra microstructure was observed under the electron micro- scope. Results Compared with the sham operated group, The S100B protein level in serum, behavioral scores and brain water content increased in the brainstem hemorrhage group at 6 hours, reached a peak at 48 hours, then decreased gradually. The S100B protein level in serum, brain water content, behavioral scores were positively correlated with each other （P 〈 0.05）. Perihematoma tissue was ob- served under the electron microscope： stellate ceils and their surrounding foot process mild swelling at 6 hours, Stellate cells visible swelling, partial cytoplasm showed vacuoles sample change, myelin part dissection, had mitochondria crest fault and vacuoles at 48 hours. Conclusions The S100B protein level in serum, brain water content, behavioral scores were positively correlated with each other. This suggests that cerebral edema may be developed through the high S100B protein level after brainstem hemorrhage.

关 键 词：脑干出血 立体定向技术 脑水肿 S-100B蛋白 

分 类 号：R743.34[医药卫生—神经病学与精神病学]