大豆北方茎溃疡病菌分离鉴定技术的改进  

An improved method for isolation and identification of northern stem canker of soybean

作　　者：华丽[1] 陈萍[1] 王卫芳[2] 黎婉芬[1] 王文祥[1] 王定国[1] 黄挺[1] 

机构地区：[1]广州出入境检验检疫局,广东广州510700 [2]广东出入境检验检疫局

出　　处：《植物检疫》2012年第2期25-27,共3页Plant Quarantine

基　　金：广东省科技厅科技计划项目(2011B020308011);广东局局内立项课题(2009GDK14)

摘　　要：本文对产生大豆北方茎溃疡病菌子囊壳的豆秆分别进行冷冻和水浸处理,发现2种处理均能促进成熟的子囊壳产孢,并且同一子囊壳经冷冻和水浸处理能够多次喷出子囊,释放子囊孢子,利用此特性有利于在不损失鉴定材料的前提下,多次获得分离物以完成病菌的分离鉴定,特别利于伴生其他真菌和子囊壳数量很少情况下,分离纯化大豆北方茎溃疡病菌。In this paper,the soybean stems which produced perithecia of northern stem canker Diaporthe phaseolorum var.caulivora were frozen or water immersed.These two treatments were both found to promote mature perithecia sporulation,and the same perithecium could spray ascus many times after frozen or water immersion.Based on this feature,we could obtain sources for isolation many times,especially benefited for isolation of northern stem canker of soybean when the stem contaminated by other species fungi or only found few perithecium.

关 键 词：大豆北方茎溃疡病菌 冷冻处理 水浸处理 分离纯化 

分 类 号：S41-30[农业科学—植物保护]