荧光原位杂交技术在急性早幼粒细胞白血病诊断及治疗中的应用价值  

Value of Fluorescence in Situ Hybridization in the Diagnosis and Treatment of Acute Promyelocytic Leukemia

作　　者：周瑞莲[1] 莫耀禧[2] 蓝梅[1] 林金盈[1] 

机构地区：[1]广西壮族自治区人民医院血液内科,南宁市530021 [2]广西壮族自治区人民医院生殖医学与遗传中心,南宁市530021

出　　处：《广西医学》2012年第3期275-278,共4页Guangxi Medical Journal

基　　金：广西医药卫生科研课题(桂卫Z2008057;桂卫Z2009133)

摘　　要：目的探讨FISH法检测PML-RARa融合基因在急性早幼粒细胞白血病(APL)中的临床应用价值。方法对21例疑诊APL患者应用FISH技术检测PML-RARa融合基因。结果 21例疑诊患者中最后确诊为APL 15例,排除APL 6例。15例APL患者中PML-RARa融合基因阳性14例,PML-RARa融合基因阴性者1例,该患者经最后确诊为APL变异型,经诱导治疗后效果不佳。6例疑诊患者PML-RARa融合基因均为阴性,确诊为急性髓细胞白血病-M2。结论 FISH法检测PML-RARa融合基因敏感、可靠,对APL的确诊及指导治疗有重要价值。Objective To investigate the application of fluorescence in situ hybridization （FISH） for the detection of PML-RARa fusion gene in the diagnosis and treatment of acute promyelocytic leukemia（APL）. Methods PML-RARa fusion gene detection was performed in 21 patients suspected of APL by FISH. Results Of the 21 suspected APL patients, fifteen cases were eonfirrned of APL. Positive PML-RARa fusion genes were found in 14 of the 15 APL patients,while one ease with negative PML-RARa fusion gene was confirmed to be variant APL and was treated with all-trans retinoie acid （ATRA）-induction therapy but the effieacy was poor. The remaining 6 suspected APL patients with negative PML-RARa fusion gene were eonfirmed to be acute myeloid leukemia-M2. Conclusion Detection of PML-RARa fusion gene by FISH is sensitive and reliable, and is of great value for the diagnosis and treatment of APL.

关 键 词：急性早幼粒细胞白血病 荧光原位杂交 PML-RARa融合基因 

分 类 号：R733.7[医药卫生—肿瘤]