单细胞悬液在人表皮生长因子受体2检测中的应用  

The application of the single cell fluid in detecting HER-2 gene or protein

作　　者：陈洪勋[1] 任兴昌[1] 

机构地区：[1]杭州市中医院病理科,浙江省杭州310007

出　　处：《中国基层医药》2012年第6期806-807,I0001,共3页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省杭州市科技发展计划项目（20090833814）

摘　　要：目的探讨利用制备的单细胞悬液建立一种简单实用的用于检测乳腺癌人表皮生长因子受体2（HER-2）基因和蛋白的实验对照方法，为病理诊断提供可靠依据。方法利用Medimachine系统将已知为HER-2阳性结果的乳腺癌组织制备成单细胞液，将其点、涂于待检测组织切片旁，分别进行HER-2的免疫组化检测和荧光原位杂交（FISH）检测。结果HER-2免疫组化结果表明乳腺癌单细胞形态完整，高倍镜下癌细胞呈现强的、完整的细胞膜棕褐着色，根据乳腺癌HER-2检测指南，可确定为HER-2阳性（＋＋＋）。同时FISH检测经计数30个细胞统计Ratio值〉2．2，提示该乳腺癌单细胞中HER-2基因发生扩增。结论经Medimachine系统制备的已知HER-2阳性的乳腺癌组织单细胞液完全可用于作为检测待检标本HER-2的阳性对照，确保每张实验切片结果的准确可靠性，且该方法简单实用，易于推广应用。Objective Using the single cell fluid to establish a kind of practical experimental method,to detect the HER-2 gene and protein of breast cancer, and to provide reliable basis for pathology diagnosis. Methods The single cell fluid of breast cancer was preparaed by Medimachine system. Sucking a little of the single cell fluid spreaded on the slide and to be examined by immunohistoehemistry using antibodies to HER-2 and fluorescence in situ hybridization. Results The cell membrane of breast cancer was positive for HER-2 by immunohistochemistry according to American society of clinical oncology of American pathologists guideline recommendations for HER-2 testing in breast cancer and the HER-2 gene of the single cell amplified obviously by fluorescence in situ hybridization which the ratio of the average of HER-2 and the average of CEP17 exceeded 2. 2. Conclusion The single cell fluid could be used as positive control when we detected the HER-2 gene or protein of breast cancer, which guaranteed the correction of the results of the sample to be detected.

关 键 词：乳腺肿瘤 表皮生长因子 细胞学技术 

分 类 号：R737.9[医药卫生—肿瘤]