实时荧光PCR检测风疹病毒的应用研究  

Study on Application of Real-time PCR in Detection of Rubella Virus

作　　者：卓菲[1] 杨贵清[1] 刘卫民[1] 吴菲菲[1] 何梅英[1] 

机构地区：[1]深圳市罗湖区疾病预防控制中心微生物检验科,广东深圳518020

出　　处：《实用预防医学》2012年第3期352-354,共3页Practical Preventive Medicine

基　　金：深圳市科技计划项目(医疗卫生类)(200803160)

摘　　要：目的建立风疹病毒的实时荧光PCR检测方法并应用于临床标本的快速检测。方法分析近四十年来流行的风疹病毒E1基因序列,设计用于病毒实时荧光PCR检测的引物和探针,建立风疹病毒实时荧光PCR检测方法。对30例临床诊断为风疹的患者标本进行实时荧光PCR检测,并与血清学检测结果进行比对。结果 30例患者标本中实时荧光PCR检测,27份阳性,3份阴性,阳性率为90.0%,远高于血清学检测(阳性率70.0%)。结论该方法具有方便快捷、特异性强、敏感性高等特点,为风疹病毒的快速诊断和分子流行病学调查提供了技术支撑。Objective To establish a real-time PCR method for detection of rubella virus,and to apply it to rapid detection of clinical samples. Methods The real-time PCR primers probes were designed by analyzing E1 gene sequences of rubella virus found in recent 40 years,and then a real-time PCR method was established for detection of rubella virus in 30 clinically diagnosed rubella patients.Real-time PCR detection results of these patients were compared with the corresponding serological detection results. Results Among 30 specimens of the patients detected by real-time PCR,there were 27 positive samples and 3 negative ones.The positive rate was 90.0%,which was significantly higher than that of serological detection method（70.0%）. Conclusions The real-time PCR detection method has the advantages of convenience,higher specificity and sensitivity.It provides technical supports for rapid diagnosis and molecular epidemiological investigation of rubella virus.

关 键 词：风疹病毒 实时荧光PCR E1基因 

分 类 号：R512.63[医药卫生—内科学]