用沙培空心菜繁殖根结线虫效果的研究  

Study on reproduction of root-knot nematodes Meloidogyne spp. inoculated to Ipomoea aquatica cultivated in sand

作　　者：焦春伟[1,2] 徐春玲[1] 谢辉[1] 王珂[1] 赵传波[1] 李宇[1] 

机构地区：[1]华南农业大学资源环境学院植物线虫研究室/植物检疫线虫检测与防疫研究中心,广东广州510642 [2]广东粤微食用菌技术有限公司,广东广州510070

出　　处：《西北农林科技大学学报（自然科学版）》2012年第3期126-130,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：公益性行业(农业)科研专项(201103018);国家香蕉产业技术体系项目(nycytx-33-06)

摘　　要：【目的】建立操作简便、高效繁殖和长期保存根结线虫的方法。【方法】通过沙培方法,于空心菜(Ip-omoea aquatica)、马铃薯(Solanum tuberosum)和番茄(Lycopersicon esculentum)移植30d时接种南方根结线虫(Meloidogyne incognita)2龄幼虫1 000条/株,同时用10盆空心菜接种爪哇根结线虫(M.javanica)和花生根结线虫(M.arenaria),测定在沙培浇灌营养液条件下,3种供试植物的根系生长以及接种繁殖根结线虫的情况。【结果】3种植物在盆钵中沙培30d后,根系生长量最大的是马铃薯,其次是空心菜,番茄最小;空心菜根系的生长期最长,达10个月。3种植物接种南方根结线虫50d后,在空心菜根系上形成的根结数、卵囊数和线虫总数分别为866.8,864.4和1 592.2,显著大于在马铃薯和番茄根系上形成的根结数(651.6和222.6)、卵囊数(643.4和234.0)和线虫总数(1 106.0和373.8)。爪哇根结线虫和花生根结线虫在沙培空心菜根系上接种后也能很好地繁殖。【结论】空心菜适应性强,在沙培浇灌营养液条件下生长周期长、易管理,适用于大量繁殖和长期保存根结线虫。[Objective] The study was done in order to establish a method for convenient, economical and mass reproduction of root-knot nematodes Meloidogyne spp.. [Method] Assays were designed and car- ried out to evaluate root growth indices of Ipornoea aquatica, Solanurn tuberosum and Lycopersicon esculen- turn inoculated with or without root-knot nematodes in sand planting. [Result] The results showed that af- ter planting the three plants in sand for 30 days,root system of S. tuberosum was the strongest,followed by the I. aquatica and that of L. esculenturn was the weakest. The root system of I. aquatica had the longest period,10 months. Inoculating 1 000 juveniles of Meloidogyne incognita each plant and letting the plants grow for another 50 days,the number of the root knobs,egg masses and total nematodes in root system of I. aquatica was 866.8,864.4 and 1 592.2 respectively, much higher than those from S. tuberosum （651.6, 643.4 and 1 106.0） and L. esculentum （222.6,234.0 and 373.8）. In addition,M, javanica and M. arenaria were also inoculated to I. aquatica roots, and the results were similar with M. incognita. [Conclusion]I. aquatica was very adapted to sand watering with nutrient fluid, and had a longer growth cycle and was easy to be controlled, so I. aquatica was a good choice to reproduce in quantity and could preserve root-knot nematodes for longer time.

关 键 词：根结线虫 繁殖 沙培培养 空心菜 番茄 马铃薯 

分 类 号：S432.45[农业科学—植物病理学] Q93-335[农业科学—农业昆虫与害虫防治]