斑贞1号、川芎嗪和氟尿嘧啶联合对人胃癌细胞多药耐药基因1表达的影响  

Effect of Ban Zhen 1,tetramethylpyrazine and fluorouracil on human gastric cancer cells multidrug resistance gene expression

作　　者：付桂珍[1] 张爱民[2] 

机构地区：[1]内蒙古自治区乌兰察布市化德县医院,内蒙古化德013350 [2]包头医学院第一附属医院消化科,内蒙古包头014010

出　　处：《中国当代医药》2012年第7期61-62,共2页China Modern Medicine

摘　　要：目的探讨斑贞1号、川芎嗪(TMP)和氟尿嘧啶(5-FU)联合逆转人胃癌细胞系SGC-7901/ADR与多药耐药基因1(MDR1)表达的相关性。方法以SGC-7901/ADR细胞为靶细胞,以不加药组为阴性对照组,氟尿嘧啶组、斑贞1号组、斑贞1号+氟尿嘧啶组、斑贞1号+氟尿嘧啶+川芎嗪(TMP)组为实验组,采用半定量RT-PCR法检测SGC-7901/ADR细胞在不同药物作用下MDR1mRNA的表达情况。结果氟尿嘧啶组与阴性对照组相比,MDR1mRNA表达差异无统计学意义(P>0.05),而其他药物组与对照组及组间比较差异有统计学意义(P<0.05),斑贞1号+氟尿嘧啶+TMP组表达量最少。结论斑贞1号、TMP和氟尿嘧啶联合逆转SGC-7901/ADR细胞耐药性可能与MDR1基因相关,为当前胃癌的治疗提供了新的理论依据。Objective To explore the correlativity of Ban Zhen 1, Tetramethylpyrazine （TMP） and fluorouracil reversing human gastric cancer cell line SGC-7901/ADR with Muhidrug resistance gene 1 （MDR1） expressions. Methods SGC- 7901/ADR cells was considered as target cells, no medicines group was considered as negative control, Fluorouracil, Banzhen-1, Banzhen-1 ＋ Fluorouracil, Banzhen-1 ＋ Fluorouracil ＋ TMP were considered as experiment groups. The expression of SGC-7901/ADR cells＂ MDR1 mRNA under different medicine groups was detected by the semi-quantitative RT- PC. Results The expression of MDR1 mRNA of Fluorouraeil was the same as negative control （P 〉 0.05）, however, the expression of MDR1 mRNA in other groups were different from each other and the group of Banzhen-1 ＋ Fluorouraeil ＋ TMP was lowest （P〈 0.05）. Conclusion It is possible correlated to MDR1 for Ban Zhen 1, TMP and Fluorouracil reversed muhidrug resistance in human gastric cancer cells, which may supply a new theoretical basis for the clinical effective treatment of gastric carcinoma.

关 键 词：胃癌 斑贞1号 川芎嗪(TMP) 氟尿嘧啶 多药耐药 多药耐药基因1(MDR1) 

分 类 号：R735.2[医药卫生—肿瘤]