模拟高原低氧兔牙周炎模型血清和牙龈组织中sICAM-1及sVCAM-1的含量  

sICAM-1 and sVCAM-1 levels in blood serum and gingival tissues in rabbits with periodontitis in analogic high altitude anoxia environment

作　　者：武曦[1] 黄镜静[1] 张纲[1] 谭颖徽[1] 高钰琪[2] 

机构地区：[1]第三军医大学新桥医院口腔科,重庆400037 [2]第三军医大学高原军事医学系

出　　处：《实用口腔医学杂志》2012年第2期178-181,共4页Journal of Practical Stomatology

基　　金：全军医药卫生科研基金资助项目(编号:06MA184)

摘　　要：目的:探讨血清和牙龈组织中可溶性细胞间黏附分子-1(sICAM-1)、可溶性血管黏附分子-1(sVCAM-1)在高原牙周病炎症反应中的作用。方法:将40只雄兔随机分为常氧对照组、常氧实验组、低氧对照组、低氧实验组,每组10只,采用正畸丝结扎下颌中切牙与牙周炎食谱的方法建立牙周炎模型,常氧组和低氧组分别在平原和模拟海拔5 000 m条件下饲养8周,对牙周组织取材进行组织学观察,用ELISA法检测各组实验动物血清和牙龈组织中sICAM-1、sVCAM-1表达情况。结果:低氧实验组sICAM-1、sVCAM-1浓度显著高于其余各组(P<0.05),且牙龈组织中浓度高于血清。结论:模拟高原低氧环境下黏附分子的表达上调,使牙周组织破坏加重,同时促使炎症反应向外周血管扩散。Objective： To investigate the expression of soluble inter-cellular adhesion molecules-1（sICAM-1） and soluble vascular adhesion molecular-1（sVCAM-1） in blood serum and gingival tissues in rabbits with periodontitis in high altitude anoxia environment.Methods： 40 male rabbits were randomly divided into four groups（n=10）： control and periodontitis groups in plain,control and periodontitis groups in plateau.The periodontitis models were established by ligation technique on the two central incisors of mandible and periodontitis diet.The anoxia groups were raised in a established condition of altitude at 5 000 meteres above sea level for eight weeks.sICAM-1 and sVCAM-1 levels in blood serum and gingival tissues were detected by ELISA.The morphology of the periodontal tissues were histologicaly observed.Results： More severe periodontitis was observed in anoxia periodontits groups.The concentrations of sICAM-1 and sVCAM-1 in anoxia periodontitis group were significantly higher than those in the other groups（P0.05）,and the levels in gingival tissues were higher in blood serum.Conclusion： Cellular adhesion molecules are upregulated in anoxia environment at high altitude and may promotes the destruction of periodontal tissues and diffusion of inflammation into the peripheral blood vessels.

关 键 词：高原 低氧 牙周炎 兔 SICAM-1 SVCAM-1 

分 类 号：R781.4[医药卫生—口腔医学]