白肋烟不同程度烟碱转化株后代烟碱转化率株间变异研究  

Study on variation of nicotine conversion rate for offsprings of burley tobacco with different conversion rates

作　　者：赵晓丹[1] 史宏志[1] 杨兴有 吴纯奎[3] 周开绪[2] 靳冬梅 

机构地区：[1]河南农业大学国家烟草栽培生理生化研究基地,郑州450002 [2]四川达州市烟草公司,达州635000 [3]四川省烟草公司,成都610000

出　　处：《中国烟草学报》2012年第1期29-34,共6页Acta Tabacaria Sinica

基　　金：四川省烟草公司2007科技项目(SCYC2007-5-3)

摘　　要：采用烟碱转化早期诱导方法,对白肋烟达所26及其自交后代不同烟株的烟碱转化率进行了分析测定,以确定烟碱转化株鉴别标准的可靠性和有效性。结果表明,烟碱转化率低于2.5%的烟株自交后代株系稳定,株间烟碱转化率变异小,且均低于2.5%,表明母代烟株为非转化株;烟碱转化率介于2.5%和3%的3个烟株自交后代均表现株间烟碱转化性状的分离,变异幅度分别为0.439%-10.331%、0.466%-21.834%和0.394%-3.757%,表明母代烟株为转化株。烟碱转化率大于3%的烟株自交后代烟碱转化率株间变异性大,烟碱转化性状分离严重;烟碱转化率大于50%的2个烟株自交后代烟碱转化率也都较高,是高转化株的表现。因此,烟碱转化率大于2.5%是确定转化株的可靠标准。Early conversion stimulation method was used to analyze alkaloid composition and content in tobacco leaves of Dasuo 26 and their selfing populations in order to verify reliability and validity. Results showed that the selfing popula- tions of plants with percent nicotine conversion （PNC） lower than 2. 5 % was stable and all had PNC lower than 2.5 % , indicating that the parent plants were non-converter. The three selfing generations from parent plants with PNC between 2.5%and 3% showed significant segregation of the trait of nicotine conversion within the range of 0. 439%-10. 331% , 0. 466%-21. 834, and 0. 394%-3. 757%, respectively, which indicated that the parents were converters. The selfing populations from parent plants with PNC greater than 3 % showed great variation for nicotine conversion, and the 2 sel- fing lines from parents with PNC greater than 50% had the highest conversion level, indicating that the parents were high converters. Therefore, PNC greater than 2.5% can be a reliable standard to determine a converter plant.

关 键 词：白肋烟 烟碱 降烟碱 烟碱转化率 鉴别标准 

分 类 号：S572[农业科学—烟草工业]