烟叶表面高效降解蛋白质芽孢杆菌的分离筛选及其鉴定  

Isolation,screen and identification of Bacillus with high proteolytic activity from tobacco leaf surfaces

作　　者：冯志珍[1] 陈太春[1] 胡喜怀[2] 郭志刚[2] 安德荣[1] 

机构地区：[1]西北农林科技大学植物保护学院,陕西省农业分子生物学重点实验室,杨凌712100 [2]陕西中烟工业有限责任公司,西安710065

出　　处：《中国烟草学报》2012年第1期101-105,共5页Acta Tabacaria Sinica

基　　金：国家“863特殊生物资源开发和利用项目”(2007AA021503);陕西中烟工业公司生物发酵增香减害项目(2010182)

摘　　要：利用芽孢杆菌耐高温的特性,从烟叶表面分离筛选并得到1株可高效降解蛋白质的菌株F-Y-11,其蛋白质降解圈直径与菌落直径比值(HC值)为5.50,蛋白酶活力为25.67 U。通过形态学特征及生理生化特征鉴定菌株F-Y-11为芽孢杆菌属。将其制成菌剂对烟丝进行发酵,测得蛋白质降解率为29.32%。A strain called F-Y-11 was isolated and screened from tobacco leaf surfaces. Its HC value, i.e. ratio of colo- ny diameter to protein degradation circle diameter, is 5.50 and proteolytic activity 25.67U. F-Y-11 strain was identified as bacillus by morphological, physiological and biochemical characteristics. Bacillus agent was prepared to ferment cut tobacco and resulted in a 29.32% of protein degradability under certain conditions.

关 键 词：烟叶 生物降解 芽孢杆菌 

分 类 号：S572[农业科学—烟草工业]