棉酚对多浪羊淋巴细胞凋亡及其对核酸降解的研究  

Degradation Effect of Gossypol on the Lymphocyte Apoptosis and Nucleic Acid of Duolang Sheep

作　　者：刘书东[1] 左文斌[1] 贺艳艳[1] 潘辉[1] 徐丽君[1] 顾海洋[1] 杨威华[2] 李莲瑞[2] 

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]塔里木大学动物科学学院,新疆兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《云南农业大学学报（自然科学版）》2012年第2期299-302,共4页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然基金项目(30960277)

摘　　要：主要通过使用RPMI 1640培养基培养淋巴细胞、瑞氏—吉姆萨染色、DNA和RNA电泳等方法来研究棉酚对多浪羊淋巴细胞的凋亡效应和对淋巴细胞核酸影响。研究结果表明,经瑞氏—吉姆萨染色4 h淋巴细胞出现凋亡小体;DNA和RNA通过琼脂糖凝胶电泳检测在4 h分别出现梯状条带和18S降解。可见,当细胞培养液中棉酚浓度为1.35μmol/L,培养淋巴细胞4 h后,可抑制多浪羊淋巴细胞的增殖,并且促进凋亡及其核酸的降解。In this study,the effects of gossypol on Duolang sheep lymphocyte apoptosis and degradation of nucleic acid on were studied by culturing lymphocytes in RPMI 1640 medium,Wright-Giemsa staining,DNA and RNA gel electrophoresis.The results showed that： lymphocyte appeared apoptotic bodies after Wright-Giemsa stained for 4 h;DNA and RNA were detected by Agarose Gel Electrophoresis.The DNA agarose gel electrophoresis showed the typical DNA ladder of apoptosis.The RNA agarose gel electrophoresis appeared 18S rRNA began to degrade at 4th hours.It was concluded that after treated by 1.35 μmol/L of gossypol for 4 h,the lymphocytes could inhibit lymphocyte proliferation of Duolang sheep,and promote apoptosis and degradation of nucleic acid.

关 键 词：棉酚 多浪羊 淋巴细胞 核酸 凋亡 

分 类 号：S826.92[农业科学—畜牧学]