氮源对Biohydrogenbacterium R3 sp.nov.发酵产氢效能影响  

Effects of nitrogen resources on fermentative biohydrogen production of Biohydrogenbacterium R3 sp.nov.

作　　者：张百慧[1] 陈红[1] 李永峰[1] 韩伟[1] 刘方婧[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《哈尔滨商业大学学报（自然科学版）》2011年第6期804-809,共6页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家863项目(2006AA05Z109);上海市重点科技攻关项目(071605122)

摘　　要：探讨有机氮源和无机氮源对高效产氢新菌种Biohydrogenbacterium R3 sp.nov.的生长和产氢代谢的影响.结果发现,有机氮源(尤其是酵母粉)对Biohydrogenbacterium R3 sp.nov.生长及产氢的促进作用比较明显,无机氮源对菌株Biohydrogenbacterium R3 sp.nov.生长和产氢量等的促进作用极小;在供试试剂中,酵母粉是提高Biohydrogenbacterium R3 sp.nov.产氢量和促进其细胞生长的最有效的氮源供应者,含有酵母粉的培养液(组Ye)中,产氢量、产氢速率和比产氢速率最大,分别为1529.69mL H2/L、16.94 mmol H2/g CDW.h和1.33 mol H2/mol葡萄糖.Hydrogen is a clean energy resource with less pollution and biohydrogen production with organic wastewater is beneficial to sustainable development.This paper discussed on the effects of nitrogen resources(include organic nitrogen resources and inorganic nitrogen resources) to hydrogen production bacterium(HPB) Biohydrogenbacterium R3 sp.nov..The promotion on cell growth and hydrogen production of organic nitrogen resource to Biohydrogenbacterium R3 sp.nov.was better than that of inorganic nitrogen resource.Yeast powder was most beneficial to promote cell growth and hydrogen production among all those nitrogen resources.The hydrogen production yield,hydrogen production ration and specific hydrogen product ration got the maximum of 1 529.69 mL H2/L,16.94 mmolH2/ g CDW·h and 1.33 mol H2/mol glucose respectively when the culture were added yeast powder(group Ye).

关 键 词：生物制氢 Biohydrogenbacterium R3 sp.nov. 有机氮源 无机氮源 

分 类 号：Q939.9[生物学—微生物学]