谷氨酰胺对溃疡性结肠炎病人结肠上皮细胞增殖的影响  

Effect of L-Glutamine on the proliferation of human colonic epithelial cells in ulcerative colitis

作　　者：翟莉莉[1] 李俊霞[1] 沈姞[1] 王化虹[1] 刘新光[1] 

机构地区：[1]北京大学第一医院消化内科,北京100034

出　　处：《肠外与肠内营养》2012年第2期71-73,77,共4页Parenteral & Enteral Nutrition

摘　　要：目的:本研究拟通过采用体外溴脱氧尿嘧啶核苷(BrdU)标记结肠增殖细胞,探讨溃疡性结肠炎(UC)结肠上皮细胞的增殖情况及L-谷氨酰胺(L-Gln)对UC病人结肠上皮增殖的影响。方法:病人行全结肠镜检查,依据2000年成都会议UC诊断标准,选择轻中度UC病人7例(UC组),设对照组5例,进行结肠组织切片,H-E染色以及标准浓度(2 mmol/L)和高浓度(4 mmol/L)L-Gln的培养液中培养,采用BrdU掺入法、免疫组化方法标记BrdU阳性结肠上皮细胞,显微镜下观察BrdU阳性结肠上皮细胞的数量。结果:UC组病人结肠上皮细胞增殖较对照组明显增强;UC组高浓度L-Gln中结肠上皮细胞增殖较标准浓度L-Gln增强。结论:UC病人结肠上皮细胞增殖增强,L-Gln能促进UC结肠上皮细胞增殖。Objectives： Ulcerative colitis（UC） is an inflammatory bowel disease of the large intestine based on disturbed proliferation and apoptosis of colonic epithelial cells.L-Glutamine（L-Gln） regulates gastrointestinal cell growth,function,and regeneration.In this study,we investigated the effect of L-Gln on the proliferation of colonic epithelial cells.Methods： Twelve biopsy specimens were obtained endoscopically from 7 patients of UC and 5 controls respectively.The specimens were used for diagnosis of UC and investigation of epithelium proliferation with markers of BrdU（Bromodeoxyuridine）.BrdU labeling of S-phase cells in colonic cells was accomplished using immunochemical detection of cells that incorporate BrdU to investigate colonic epithelial proliferation and the effect of L-Gln on it.Results： Cell Proliferation was significantly increased in UC compared to controls（P0.05）.In UC,after incubating for 4 hours,proliferation increased significantly in higher concentration（4 mmol/L） of L-Gln compared with the standard concentration（2 mmol/L）（P0.05）.Conclusion： In UC,L-Gln increases the proliferation of colonic epithelial cells.

关 键 词：溃疡性结肠炎 增殖 溴脱氧尿嘧啶核苷 谷氨酰胺 

分 类 号：R574.62[医药卫生—消化系统]