香港巨牡蛎♀×太平洋牡蛎♂异源三倍体的初步研究  

PRIMARY STUDY ON ALLOTRIPLOID INDUCTION OF CRASSOSTREA HONGKONGENSIS ♀×C. GIGAS ♂

作　　者：刘剑[1] 王昭萍[1] 孔静[1] 于瑞海[1] 王有廷 王廷军 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]蓬莱海益水产有限公司,山东烟台265600

出　　处：《海洋湖沼通报》2012年第1期33-40,共8页Transactions of Oceanology and Limnology

基　　金：国家重点基础研究发展计划项目(2010CB126406);国家自然科学基金项目(30771622);国家高技术研究发展规划项目(2006AA10A401)资助

摘　　要：本文以香港巨牡蛎(Crassostrea hongkongensis)和太平洋牡蛎(C.gigas)为研究对象,进行牡蛎异源三倍体研究试验。在水温25℃条件下,香港巨牡蛎剥离卵子于海水中浸泡促熟1.5h后,与太平洋牡蛎精子受精,获得最高卵裂率为(24.40±3.79)%。受精后20min,采用盐度为10的低渗海水处理杂交受精卵25min,可获得68.65%的异源三倍体幼虫,孵化率为12.71%。异源三倍体幼虫的存活率和生长率均未表现出优势。受精后第9天,异源三倍体幼虫的存活率仅为(0.116±0.023)%,平均壳长为(103.50±0.85)(m;对照组的存活率和壳长分别为(42.17±2.74)%和(123.25±8.60)μm。幼虫在附着变态前死亡。Prior to the allotriploid induction, the feasibility of fertilization between Cras-sostrea hongkongensis （Lain and Morton, 2003） eggs and C. gigas （Thunberg, 1973） sperm had been verified before. Results showed that cleavage rate of （24.40±3.79）% of the inter-specific hybrid can be received with the eggs immersed in 25℃ seawater for 1.5h. The zy-gotes treated for 25 rain with a hypotonic solution of 10 salinities at 20rain after fertilization can yield the highest triploidy rate of 68.65% and the hatchery rate of 12.71%. No statisti-cal advantages of growth rate and survival rate were found in allotriploid group compared with control group. The survival rate and shell length of allotriploid group were （0. 116±0. 023）% and （103.50±0.85）ptm at the 9th day after and shell length of control group were （42.17±2. riploid hybrid larvae died before metamorphism. insemination. However, the survival rate 74）% and （123.25±8.60）ttm. All allot-

关 键 词：牡蛎 异源三倍体 低渗诱导 香港巨牡蛎 太平洋牡蛎 

分 类 号：S968.3[农业科学—水产养殖]