细胞外ATP对U87胶质瘤细胞生长和侵袭的作用  

Extracellular adenosine triphosphate improves growth and invasion of U87 glioma cells

作　　者：王晖[1] 冯华[1] 陈图南[1] 赵明月[1] 李博[1] 李飞[1] 

机构地区：[1]第三军医大学西南医院神经外科,全军神经系统疾病微创诊治专科中心,全军神经外科神经创伤防治重点实验室,重庆400038

出　　处：《第三军医大学学报》2012年第7期589-592,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展计划(“973”计划,2010CB529403);“十一五”国家科技支撑计划(2007DAI05D08);国家自然科学基金(30973494)~~

摘　　要：目的观察细胞外三磷酸腺苷(adenosine Triphosphate,ATP)对体外培养的U87人脑胶质瘤细胞增殖、侵袭和迁移能力的影响。方法采用MTT法检测不同浓度细胞外ATP对U87胶质瘤细胞增殖的影响;以相同条件下不加ATP作为对照组,以加入100μmol/L ATP作为处理组,采用Transwell细胞侵袭实验检测100μmol/L细胞外ATP对U87胶质瘤细胞侵袭能力的影响;用时间显微镜动态观察100μmol/L的ATP作用下U87胶质瘤细胞的迁移情况。结果高浓度(5 000μmol/L)细胞外ATP对U87胶质瘤细胞增殖有显著抑制作用,较低浓度(50、100、500μmol/L)细胞外ATP对U87胶质瘤细胞增殖无明显影响,5 000μmol/L ATP组与其他浓度组比较,差异有统计学意义(P<0.05);Transwell侵袭实验中,处理组细胞跨膜细胞数为(163.83±17.81)明显多于对照组(89.83±13.27)(P<0.01);迁移实验中,处理组细胞运动速度为(163.83±17.81)μm/h,对照组细胞运动速度为(89.83±13.27)μm/h,2组比较,差异有统计学意义(P<0.05)。结论 100μmol/L的细胞外ATP对体外培养的U87胶质瘤细胞的侵袭和迁移具有明显促进作用。Objectives To determine the effect of extracellular adenosine triphosphate（ATP） on the growth and invasion of U87 glioma cells in vitro.Methods Cell counting kit-8 was used to detect the proliferation in U87 cells after the treatment of ATP at different concentrations of 0,50,100,200,500 and 5 000 μmol/L.Invasion and migration of U87 cells with 100 μmol/L ATP treatment were examined by Transwell and Time microscopy.Results The proliferation of U87 glioma was inhibited obviously by high concentration of ATP（5 000 μmol/L）.However,there was no significant impact on the proliferation in U87 cells by a lower amount of extracellular ATP（50,100 and 500 μmol/L）.The invasion and migration ability of U87 cells were significantly enhanced by the using of 100 μmol/L ATP.Conclusion Extracellular ATP can significantly enhance the ability of invasion and migration in U87 cells.

关 键 词：ATP 胶质瘤 侵袭 迁移 

分 类 号：R730.23[医药卫生—肿瘤] R730.264[医药卫生—临床医学]