辽宁海城小孤山披毛犀化石的古DNA分析  

Ancient DNA Analysis of Woolly Rhinoceros in Xiaogu Mountain,Haicheng City,Liaoning Province

作　　者：双小燕[1] 袁俊霞[1] 侯新东[1] 盛桂莲[1] 伊剑[1] 赖旭龙[1] 

机构地区：[1]中国地质大学生物地质与环境地质国家重点实验室,武汉430074

出　　处：《地质科技情报》2012年第2期40-44,共5页Geological Science and Technology Information

基　　金：国家自然科学基金项目(40672006;40902008);中国国家外事专家局"111"项目(B08030);973项目(2011CB808800);中国科学院脊椎动物进化系统学重点实验室开放课题基金项目(2010LESV009)

摘　　要：从采自辽宁省海城县小孤山晚更新世披毛犀化石样品中成功地获得了1 080bp细胞色素b基因序列,调用Genbank中已发表的披毛犀序列及5种现生犀牛的同源序列,以马作为外类群,采用邻接法(NJ法)和最大似然法(ME法)构建系统发育树,结果均显示披毛犀与现生苏门答腊犀亲缘关系最近,且辽宁省海城县小孤山样品处于披毛犀分支的根部;对披毛犀样品进行基因分异度分析结果表明,小孤山的披毛犀样品具有较高的基因分异度,为探讨披毛犀的起源、迁徙及演化等问题提供了重要的分子依据。In this study,1 080 bp sequence of mitochondrial cytochrome b gene was successfully retrieved from a Late Pleistocene wooly rhinoceros fossil excavated from Xiaogu Mountain,Haicheng City,Liaoning Province.Analysis of phylogenetic tree with horse as out group showed the same topological of phylogenetic trees using Neighbor-Joining and Maximum likelihood methods.Our results suggested that woolly rhinoceros has relatively close relationship with extant sumatran rhinoceros.Furthermore,the phylogenetic position of Xiaogu Mountain sample was always at the root of this branch.Finally,our data revealed that the woolly rhinoceros from China showed higher gene divergency than European and Northern Asian counterparts.Our study provides molecular evidence for the origin and extinct of woolly rhinoceros.

关 键 词：披毛犀 古DNA 系统发育分析 晚更新世 基因分异度 

分 类 号：P534.63[天文地球—第四纪地质学] Q915.87[天文地球—古生物学与地层学]