肝癌组织RUNX3mRNA和蛋白表达及其与临床病理的相关性研究  

The associated research on the expression of RUNX3 mRNA and protein in hepatic cell carcinoma as well as the relationship with clinical pathological parameters

作　　者：卢燕辉[1] 郑瑞丹[1] 徐成润[1] 陈洁[1] 伍伟平[1] 陈碧芬[1] 李建国[2] 林志川[2] 

机构地区：[1]福建漳州解放军第175医院厦门大学附属东南医院肝病治疗中心,漳州363000 [2]福建医科大学附属漳州市医院肝胆外科

出　　处：《中华实验和临床病毒学杂志》2011年第6期441-444,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：南京军区医学科技创新课题（10MA075）

摘　　要：目的 探讨肝癌组织及其癌旁组织中RUNX3mRNA和蛋白表达情况,并分析其与临床病理因素的相关性.方法 采用RT-PCR（逆转录-聚合酶链反应）和IHC（免疫组织化学）分别检测肝癌组织及其癌旁组织中RUNX3mRNA和蛋白表达水平,并分析与临床病理因素的相关性.结果在51例肝癌组织中RUNX3mRNA表达量相对值为：0.4509±0.0963；在51例相对应的癌旁组织中RUNX3mRNA表达量相对值为：0.9147 ±0.0222；两者间差异有统计学意义（t=33.6087,P＜0.001）.在51例肝癌组织中RUNX3蛋白表达阳性率为49.02％（ 25/51）；而在51例相对应的癌旁组织中RUNX3蛋白表达阳性率为82.35％ （42/51）；两者间差异有统计学意义（x2=12.5706,P＜0.005）.RUNX3mRNA和蛋白表达水平均在分化程度、门静脉癌栓、肝内转移等病理因素差异有统计学意义（P＜0.05）,而在性别、肿瘤直径、肿瘤部位、癌肿出血坏死、组织分型的差异均无统计学意义（P＞0.05）.结论 RUNX3基因在肝癌组织中mRNA和蛋白表达水平均显著低于在癌旁组织中的表达水平,RUNX3mRNA和蛋白表达下调可能在肝癌的发生、发展过程中起着重要作用；RUNX3基因可能是肝癌的一种抑癌基因.Objective To investigate the expression of RUNX3 mRNA and protein in hepatic cell carcinoma （HCC）and surrounding normal tissue,to analyze the relationship between RUNX3 expression and clinical pathological parameters.Methods Reverse transcription polymerase chain reaction （ RT-PCR ） and immunohistochemistry（IHC） were performed to detect the expression of RUNX3 mRNA and protein in HCC and surrounding normal tissue respectively,and their relationship with clinical pathological parameters were analyzed.Results The relative expression value of RUNX3 mRNA found in 51 cases HCC was 0.4509 ± 0.0963,and that did in 51 cases surrounding normal tissue was 0.9147 ±0.0222.The difference of RUNX3 mRNA expression between two kinds of samples was statistically significant（ t =33.6087,P 〈 0.001 ）.The positives rate of RUNX3 protein expression found in 51 cases HCC tissue was 49.02% （25/51）and that did in 51 cases surrounding normal tissue was 82.35% （42/51）.The difference of RUNX3 protein expression between two kinds of samples was statistically significant （ x2 =12.5706,P 〈 0.005 ）.The difference of RUNX3 mRNA and protein expression in some clinical pathological parameters involving differentiation degree,invasion,cancer thrombus and diversion in liver were statistically significant （P 〈 0.05 ）.However that were not in another clinical pathological parameters involving gender,cancer diameter,cancer location as well as hemorrhage and necrosis of cancer,histotype （ P 〉 0.05 ）.Conclusion The expression of RUNX3 mRNA and protein in HCC were significantly lower than that in surrounding normal tissue. The lower expression of runx3 protein in the HCC probably plays an important role in the tumorigenesis and development of HCC.The RUNX3 gene may be an anti-oncogene of HCC.

关 键 词：RNA 信使 蛋白质RUNX3 肝肿瘤 逆转录-聚合酶链反应 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]