首乌藤多糖提取方法比较  

Comparison of Extraction Method of Caulis Polygoni Multiflori Polysaccharide

作　　者：于雷[1] 王保奇[1] 董晶晶[1] 杨云[1] 

机构地区：[1]河南中医学院药学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第7期38-40,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：郑州市金水区科技攻关项目(20093534)

摘　　要：目的:比较不同提取方法对首乌藤多糖提取效率的影响。方法:采用苯酚-硫酸法测定多糖含量,以粗多糖得率和多糖质量分数为评价指标,对首乌藤多糖不同提取方法进行研究。结果:6种方法中以氢氧化钠提取方法的效果最优,粗多糖得率和多糖质量分数可达25.13%和21.46%。结论:氢氧化钠提取首乌藤多糖是一种效率高且稳定可行的提取方法。Objective：To compare effect of different extraction methods to extraction efficiency of polysaccharide from Caulis Polygoni Multiflori.Method：The content of polysaccharide was determined by phenol-sulfuric acid method,study on different extraction methods for polysaccharide from Caulis Polygoni Multiflori with the content and yield of polysaccharide as indexes.Result：Sodiumhydroxide extraction method was the best by comparing with other five methods,yield and the content of polysaccharide were up to 25.13%,21.46%,respectively.Conclusion：Sodiumhydroxide extraction was proved to be a high efficiency and stable method for extraction of polysaccharide from Caulis Polygoni Multiflori.

关 键 词：首乌藤多糖 水提 碱提 酶提 酶-水提 

分 类 号：R283.6[医药卫生—中药学]