MADD在肺腺癌组织中的表达及对肺腺癌A549细胞增殖和凋亡的影响  

Expression of MADD in lung adenocarcinoma tissues and its effects on proliferation and apoptosis of lung adenocarcinoma A549 cells

作　　者：魏玉平[1] 吴金香[2] 郭元芳[1] 孙高英[1] 张强[2] 毕文祥[1] 董亮[2] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,山东济南250012 [2]山东大学齐鲁医院呼吸内科,山东济南250012

出　　处：《细胞与分子免疫学杂志》2012年第4期354-357,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:探讨MADD在肺正常组织及肺腺癌组织中的表达及其对肺腺癌细胞增殖和凋亡的影响。方法:收集肺临床病理组织标本,免疫组化法检测肺正常组织和肺癌组织MADD表达;培养人肺腺癌A549细胞,逆转录PCR检测其IG20基因表达;用携带MADD基因的质粒和能够沉默MADD表达的慢病毒载体分别转染A549细胞,Western blot、MTT分析和流式细胞术检测其MADD表达、增殖及凋亡。结果:肺腺癌组织MADD表达水平明显高于肺正常组织和肺鳞癌组织;A549细胞能够表达MADD;高表达MADD能抑制A549细胞凋亡,提高其增殖活力,而沉默MADD表达则能促进A549细胞凋亡,降低其增殖活力。结论:肺腺癌组织MADD表达明显增高;MADD可通过抑制凋亡来促进肺腺癌细胞生存。AIM： To investigate the expression of MAPK-activating death domain protein （MADD） in lung adenocarcinoma tissues and its effects on proliferation and apoptosis of lung adenocarcinoma A549 cells. METHODS： Immunohistochemistry was used to detect the expression of MADD in lung normal and tumor tissues. The expression of IG20 gene in A549 cells was measured by reverse transcription polymerase chain reaction. A549 cells were transfected with pEYFP-MADD plasmids carrying MADD gene or pNL-SIN-GFP-MID lentiviral vectors used for RNA interference, MADD expression and cell proliferation and apoptosis were determined by Western blot, MTT assay, and flow cytometry. RESULTS： The expression levels of MADD were higher in lung adenocarcinoma and squamous cell carcinoma tissues than that in lung normal tissues, and lung adenocarcinoma tissues expressed more MADD than lung squamous cell carcinoma tissues. The transcript encoding MADD was expressed in A549 cells. The transfection of pEYFP-MADD plasmids could increase MADD expression and cell proliferation of A549 cells, while the A549 cells transfected with pNL-SIN-GFP-MID Ientiviral vectors showed significantly decreases in the MADD level and proliferation. It is shown that MADD overexpression could inhibit A549 cell apoptosis, and knock down of MADD could promote apoptosis of them. CONCLUSION： The expression of MADD

关 键 词：肺癌 MADD 免疫组化 细胞凋亡 TRAIL 

分 类 号：R734.2[医药卫生—肿瘤]