Ⅱa类乳酸菌细菌素的异源表达研究进展  

Research Advances in Heterologous Expression of ClassⅡa Bacteriocins from Lactic Acid Bacteria

作　　者：刘国荣[1,3] 孙勇 李平兰[3] 

机构地区：[1]北京工商大学食品添加剂与配料北京高校工程研究中心,食品风味化学北京重点实验室,北京100048 [2]北京市食品研究所,北京100162 [3]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2012年第5期323-327,共5页Food Science

基　　金：北京工商大学青年教师科研启动基金项目(QNJJ2011-042);国家自然科学基金项目(31071591)

摘　　要：Ⅱa类乳酸菌细菌素由于其对单核细胞增生李斯特菌的强烈抑菌活性,已成为天然食品防腐剂研究与开发的热点。但是受生物合成调控系统控制,天然细菌素的产量往往很低而且提取过程较为复杂,很难满足相关研究和实际应用的需求。为此,许多研究者进行过Ⅱa类细菌素的异源表达研究,本文对该类细菌素在大肠杆菌、乳酸菌以及酵母菌中的异源表达研究作较为全面系统的综述,并指出目前存在的主要问题及今后的研究方向。Class Ⅱ a bacteriocins from lactic acid bacteria, which have a strong antibacterial activity against Listeria monocytogenes, have become a hot topic in the research and development of natural preservatives. However, the bacteriocins are always produced at very low levels under the control of the biosynthesis regulatory system and their extraction is very complex, which makes it very difficult to meet the demands for relevant studies and practical applications. For this reason, the heterog- enous expression of class Ⅱ a bacteriocins has been widely studied in recent years. This paper summarizes a comprehensive systematic review of recent studies on the heterogenous expression of the bacteriocins in E. coli, lactic acid bacteria and yeast and points out the current main problems and future research directions.

关 键 词：Ⅱa类乳酸菌细菌素 单核细胞增生李斯特菌 异源表达 

分 类 号：Q939.117[生物学—微生物学]