南蛇藤内生真菌的分离及抑菌活性初探  

Preliminary Study on the Isolation and Fungicidal Activities of Endophytic Fungi from Celastrus orbiculatus Thunb

作　　者：田小曼[1] 许蒙 

机构地区：[1]杨凌职业技术学院,陕西杨凌712100 [2]陕西咸阳市秦都区植保站,陕西咸阳712000

出　　处：《西北农业学报》2011年第11期183-187,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省攻关项目(2008K01-01)

摘　　要：采用常规方法从南蛇藤的叶与果实中分离得到21株内生真菌,以PDB培养基对其进行液体培养,用高压蒸汽灭菌过的发酵处理液和丙酮粗提物对小麦赤霉菌、棉花枯萎菌、番茄叶霉菌等8种病原菌进行抑菌活性测定。结果表明,抑菌活性成分主要存在于丙酮粗提物中。筛选出1株抗菌谱较广的内生真菌Y9,其对番茄灰霉病菌、番茄早疫病菌、苹果炭疽病菌、西瓜枯萎病菌、苹果褐腐病菌、小麦赤霉病菌6种病原菌有较好抑菌活性。Twenty-one endophytic fungi strains isolated from the leaves and fruits of Celastrus orbiculatus by traditional tissue isolation method were cultivated in PDB（Potato Dextrose Broth） medium.The antifungal activities of the fermentation broth and the acetone extracts of mycelia against 8 plant pathogenic fungi were tested through mycelium growth rate.The results showed that the compounds of fungicidal activity mostly existed in the acetone extracts of mycelia.Y9 strain had higher antifungal activity on six pathogenic fungi including Botrytis cinerea,Alternaria solani,Glomerella cingulata,Fusarium oxysporum,Sclerotinia frucligena,and Fusarium graminearum.

关 键 词：南蛇藤 内生真菌 抑菌活性 

分 类 号：S482.2[农业科学—农药学]