不同介质中大肠杆菌碱性磷酸酶构象变化的研究  

Conformational Change of Escherichia Coli Alkaline Phosphatase in Various Media

作　　者：张海容[1] 晋卫军[2] 董川[2] 刘长松[2] 

机构地区：[1]山西省忻州高等专科学校化学系 [2]山西大学化学系,太原030006

出　　处：《分析化学》2000年第1期57-60,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金!29575204;29875016

摘　　要：利用色氨酸残基作为内源荧光探针在膜模拟剂（各种表面活性剂）和不同变性剂中对大肠杆菌碱性磷酸酶（AP）的构象变化进行了系统的研究。通过测定在不同变性时间下盐酸肌浓度对荧光强度的影响以及荧光强度随pH有规律的变化，进一步证实了该蛋白质变性过程中形成较稳定中间态的结论。The conformational change of Escherichia coli alkaline phosphatase (AP) was systematcally studied using tryptophan residues as an intrinsic fluorescent probe in various media, such as anionic type surfactant of sodium dodecyl sulfate(SDS), cationic type of cetyltrimethylammonium bromide (CTMAB),and nonionic type of polyoxyethylene (23 ) dodecanol (Brij-35 ), guanidine hydrochloride (Gdn· HCl),urea and acid. A regular change in fluorescence intensity at differen time could be determined due to the change of concentration of Cdn· HCl or low pH. The conclusion was further confirmed that the protein formed a stable intermediates state in the denatured processes.

关 键 词：碱性磷酸酶 色氨酸残基 蛋白质构象 荧光探针 AP 

分 类 号：O629[理学—有机化学]