半边旗提取物5F对人结肠腺癌细胞株SW620埃兹蛋白表达及活性影响  

Effect of Pteris Semipinnata L Extract on Ezrin Expression and Activity in Human Colon Adenocarcinoma SW620 Cells

作　　者：陈建发 陈引香 李萍 傅明 吕应年[2] 

机构地区：[1]解放军422中心医院普外科,广东湛江524009 [2]广东医学院广东天然药物研究与开发重点实验室

出　　处：《华南国防医学杂志》2011年第4期298-300,304,共4页Military Medical Journal of South China

基　　金：国家自然科学基金(3987099)

摘　　要：目的观察半边旗提取物5F对人结肠腺癌细胞株SW620的埃兹(Ezrin)蛋白表达及活性影响。方法采用四氮甲唑蓝[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT]检测5F对SW620细胞的增殖抑制作用,采用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测Ezrin mRNA,采用Western blot法检测Ezrin和磷酸化Ezrin蛋白的表达。结果 5F抑制SW620细胞增殖作用呈剂量和时间依赖性,其48h半数抑制量(50%concentrction of inhibition,IC50)为0.968mg/ml。随着5F干预浓度增加,Ezrin的表达被抑制;2.0mg/ml5F干预后,Ezrin和磷酸化Ezrin蛋白表达的抑制作用明显增强,与对照组比较差异均有统计学意义。结论半边旗提取物5F在体外能抑制SW620细胞的增殖,通过抑制Ezrin的表达,抑制大肠癌侵袭转移。Objective To investigate the effect of Pteris Semipinnata L extract（5F） on the expression and activity of Ezrin in the human colon adenocarcinoma SW620 cells.Methods Effect of the extract on the proliferation of the human colon adenocarcinoma SW620 cells was assessed using 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assays.Ezrin mRNA was determined by reverse transcription-polymerase chain reaction（RT-PCR）,and the expression of Ezrin was analyzed with Western Blot assay.Results The inhibitory effect of the extract on the proliferation of SW620 cells was dose-and time-dependent,and its IC50 at 48 hours was 0.968 mg/ml.With the increase of the 5F concentration,the expression of Ezrin mRNA was inhibited.When treated with 2.0 mg/ml 5F,the expression levels of Ezrin and its phosphorylation proteins were significantly inhibited compared with the control group（P〈0.05）.ConclusionPteris Semipinnata L extract 5F can inhibit the proliferation of SW620 cells and the nvation and metabasis of colon adenocarcinoma by inhibiting Ezrin expression.

关 键 词：5F 埃兹蛋白 大肠癌 SW620 半边旗 

分 类 号：R735.35[医药卫生—肿瘤]