CIK细胞对急性髓细胞白血病微小残留病作用的研究  

Study on effect of CIK cell on minimal residual disease in acute myelocytic leukemia

作　　者：程永贵[1] 杨广民[1] 肖中平[1] 

机构地区：[1]吉林省人民医院检验科,吉林长春130021

出　　处：《吉林医学》2012年第8期1571-1574,共4页Jilin Medical Journal

摘　　要：目的:制备和观察CIK细胞对自体、异体原代急性髓细胞白血病(AML)细胞的杀伤作用和对G0期CD3+4白血病细胞的细胞毒作用,以及对正常骨髓造血干细胞的影响。方法:取AML化疗后及正常人外周血单个核细胞加入一系列细胞因子诱导培养CIK细胞,流式细胞仪检测CIK细胞表型,G带法分析CIK细胞核型。冻存、复苏后作为靶细胞,MTT法测定CIK细胞对自体、异体原代白血病细胞杀伤作用。AO染色法测定复苏后G0期AMLCD3+4细胞比例。流式细胞仪检测CIK细胞杀伤前后G0期CD3+4细胞比例变化。半固体培养法检测CIK细胞对正常骨髓CFU-GM、BFU-E集落形成的影响。结果:CIK细胞可从白血病患者外周血中获得,来源于正常淋巴细胞,对自体原代白血病细胞有明显杀伤作用,对G0期白血病细胞有明显抑制作用,对正常造血干细胞无杀伤作用。Objective To prepare and observe the damaging effect of CIK cell on autogeneic and xenogenic primary acute myelocytic leukemia（AML） cell and cytotoxic function to CD＋34 leukemia cell in G0 stage,as well as the influence to normal marrow hemopoietic stem cell.Method The mononuclearcell in peripheral blood from chemotherapied AML and the normal persons was added a series of cell factor to culture the CIK cell,the CIK phenotype was examined with flow cytometer,the CIK cell nuclear type was analyzed with the G belt method.We saved and took them as the target cell after frozen,the damaging effect of CIK cell on autogeneic and xenogenic primary AML cell was determined with the MTT method.AML CD＋34 cell proportion in G0 stage after recovery was determined with AO dyeing method.CD＋34 cell proportion changes in G0 stage before and after killing by CIK cell was examined with flow cytometer.The influence of CIK cell on normal marrow CFU-GM and BFU-E colony forms was examined with semi-solid culture method.Results CIK cell could be obtained from peripheral blood of the leukemia patients,which was originated from the normal lymphocyte,had the obvious damaging effect on autogeneic primary leukemia cell,had obvious inhibitory action on leukemia cell in G0 stage,had no damaging effect on normal hemopoietic stem cell.Conclusion CIK cell can be as the effective tool to eliminate the residual leukemia cell.

关 键 词：白血病 微小残留病 CIK细胞 

分 类 号：R733.7[医药卫生—肿瘤]