磷酸二酯酶4D基因rs33395位点与维吾尔族和汉族缺血性脑卒中的相关性研究  

Relationship between rs33395 of Phosphodiesterase 4D Gene and Ischemic Stroke in Uygur and Han Population

作　　者：张小宁[1] 杜合宾[1] 王静[1] 马建华[1] 

机构地区：[1]新疆医科大学第一附属医院神经内科,830054

出　　处：《中国临床神经科学》2012年第2期135-139,共5页Chinese Journal of Clinical Neurosciences

基　　金：国家自然科学基金(编号:81060097)

摘　　要：目的:探讨中国维吾尔族和汉族人群中,磷酸二酯酶4D(pde4d)基因rs33395位点的多态性与缺血性脑卒中的相关性。方法:采用PCR限制性片段长度多态性(PCR-RFLP)和基因测序方法检测病例组(226例缺血性脑卒中患者,其中维吾尔族110例、汉族116例)和对照组(220例无神经系统疾病的患者,其中维吾尔族102例、汉族118例)的pde4d基因rs33395多态性。并对各组基因型分布和等位基因频率进行比较。结果:在病例组和对照组中,CT基因型分布频率最高,T等位基因分布频率高于C等位基因;但各组中,维吾尔族与汉族两民族间及同民族内部基因型和等位基因频率的分布均差异无统计学意义(P>0.05)。结论:pde4d基因rs33395可能与维吾尔族、汉族缺血性脑卒中无相关性。Aim： To investigate the relationship between rs33395 of phosphodiesterase 4D（pde4d） gene polymorphism and ischemic stroke（IS） in Uygur people and Han people.Methods： The polymorphism of SNP87 in pde4d gene were detected by using PCR restriction fragment length polymorphism（PCR-RFLP） and gene sequencing method in 226 patients with ischemic stroke patients（110 Uygur people and 116 Han people in case group） and 220 patients without neurological disease（102 Uygur people and 118 Han people in the control group）.The genotype distribution and allele frequency of each group were compared.Results： Although the distribution frequency of CT genotype was maximum and the distribution frequency of T allele was higher than that of C allele in both of the case group and the control group,there was no significant statistical differences between them（P0.05）.And there was no significant statistical differences among Uygur,Han people and each other（P0.05）.Conclusion： The rs33395 of pde4d gene may not be associated with ischemic stroke in Uygur and Han population.

关 键 词：磷酸二酯酶4D 单核苷酸多态性 缺血性脑卒中 汉族 维吾尔族 

分 类 号：R743[医药卫生—神经病学与精神病学]