"Click chemistry" preparation of WCX packings for protein separation  

作　　者：Kai Lou Zhao Chao Song Fei Wang Quan Bai 

机构地区：[1]Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education,Institute of Modern Separation Science,Northwest University,Xi'an 710069,China

出　　处：《Chinese Chemical Letters》2012年第3期305-308,共4页中国化学快报（英文版）

基　　金：supported by the National 863 Program(No2006AA02Z227);the Natural Science Fondation of Shaanxi Province(No2011JZ002);the National Fund for Fostering Talents in Basic Science(NoJ0830417)

摘　　要：Click chemistry was applied to immobilize three kinds of alkyne-carboxylic acids onto azide-modified silica gel to prepare three novel stationary phases for weak cation exchange chromatography（WCX）.The developed protocol combines the benefits of operational simplicity,exceptionally mild conditions and high surface loadings.Six kinds of standard proteins were separated completely on the novel packings.Compared with commercial WCX columns,the three kinds of novel WCX packings prepared by click chemistry approach have better resolution and selectivity.Lysozyme was purified successfully from egg white with the novel WCX column by one step.The purity was more than 97%and a high specific activity was achieved to be 81,435 U/mg.The results illustrate the potential of click chemistry for preparation of stationary phase for IEC.Click chemistry was applied to immobilize three kinds of alkyne-carboxylic acids onto azide-modified silica gel to prepare three novel stationary phases for weak cation exchange chromatography（WCX）.The developed protocol combines the benefits of operational simplicity,exceptionally mild conditions and high surface loadings.Six kinds of standard proteins were separated completely on the novel packings.Compared with commercial WCX columns,the three kinds of novel WCX packings prepared by click chemistry approach have better resolution and selectivity.Lysozyme was purified successfully from egg white with the novel WCX column by one step.The purity was more than 97%and a high specific activity was achieved to be 81,435 U/mg.The results illustrate the potential of click chemistry for preparation of stationary phase for IEC.

关 键 词：Click chemistry Stationary phase Ion exchange chromatography PROTEIN SEPARATION 

分 类 号：TQ127.12[化学工程—无机化工] Q510.3[生物学—生物化学]