Study of STAT3 G-quadruplex folding patterns by CD spectroscopy and molecular modeling  

作　　者：Sen Lin Ming Xu Gu Yuan 

机构地区：[1]Beijing National Laboratory for Molecular Sciences(BNLMS),Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China [2]Key Laboratory of Molecular Cardiovascular Sciences of Ministry of Education,Institute of Vascular Medicine,Third Hospital,Peking University,Beijing 100191,China

出　　处：《Chinese Chemical Letters》2012年第3期329-331,共3页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos90913004 and 30821001);the Natural Science Foundation of Beijing(No7082101);Program for New Century Excellent Talents in University;Beijing Talents Foundation Beijing National Laboratory for Molecular Sciences

摘　　要：The G-quadruplexes formed from G-rich strands in the telomere and oncogene-promoter regions are regarded as new promising targets in the cancer therapy.A G-quadruplex in the downstream flanking region of the signal transducer and activator of transcription 3（STAT3） gene was explored.Its folding patterns were proposed to be 3：2：2 and 3：3：1 loop isomers by the mutation analysis by CD spectroscopy.The structures were constructed and refined by molecular modeling method.The G-quadruplexes formed from G-rich strands in the telomere and oncogene-promoter regions are regarded as new promising targets in the cancer therapy.A G-quadruplex in the downstream flanking region of the signal transducer and activator of transcription 3（STAT3） gene was explored.Its folding patterns were proposed to be 3：2：2 and 3：3：1 loop isomers by the mutation analysis by CD spectroscopy.The structures were constructed and refined by molecular modeling method.

关 键 词：G-QUADRUPLEX STAT3 gene CD spectroscopy Molecular modeling 

分 类 号：Q756[生物学—分子生物学] TQ424.25[化学工程]