冬凌草甲素通过改变CyclinD2、CyclinE、P^27的表达对PC-3细胞抑制增殖和凋亡诱导效应  

Effects of Oridonin on proliferation and apoptosis of PC-3 cells

作　　者：李进 杨罗艳[2] 吴洪涛[2] 

机构地区：[1]湘潭市中心医院泌尿外科,湘潭411100 [2]中南大学湘雅二医院泌尿外科

出　　处：《中国男科学杂志》2012年第2期11-14,共4页Chinese Journal of Andrology

摘　　要：目的探索冬凌草甲素对人雄激素非依赖性前列腺癌细胞株——PC-3细胞的诱导凋亡作用及其分子机制。方法用不同浓度的冬凌草甲素干预PC-3细胞，MTT试验分析观察其对PC-3细胞活力的影响；流式细胞仪检测细胞周期变化；实时荧光定量PCR方法检测PC-3细胞CyclinD2、CyclinE、P2，蛋白表达的变化。结果（1）冬凌草甲素增加G0／G1期PC．3细胞百分率，降低S期PC-3细胞百分率；（2）冬凌草甲素以浓度依赖性方式抑制PC-3细胞的CyclinD2、CyclinE蛋白表达，而P^27，蛋白表达上调；结论冬凌草甲素通过影响细胞周期调节蛋白、阻断细胞周期G1／S期“稽查点”：抑制CyclinD2、CyclinE，上调P^27等途径抑制PC-3细胞增殖及诱导PC-3细胞凋亡。Objective To investigate the effect of Oridonin on apoptosis of human prostate cancer cell lines （PC-3 cells） and their molecular mechanism. Methods The PC-3 cells were intervened by Oridonin in different concentration. The vitality of the PC-3 cells was detected by MTT assay. The change of cell cycle was analyzed by the flow cytometry; and the changes of expressions of CyclinD2, CyclinE, p27 in PC-3 cells were detected by the real-time fluorescent quantitative determination. Results （1） Oridonin increased the percentage of the G0/G1 phase and decreased the S phase of PC-3 cells; （2） Oridonin down regulated the expression of CyclinD2 and CyclinE, and up regulated the expression of p27 in a concentration-dependent way in PC-3 cells. Conclusion Oridonin can inhibit proliferation of PC-3 cells and induce their apoptosis through regulating the cell cycle protein, blocking the ＂checkpoint＂ of the G1/S phase, down-regulating CyclinD2 and CyclinE as well as up-regulating P^27.

关 键 词：前列腺肿瘤 冬凌草素 细胞增殖 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]