塞来昔布对直肠癌HCA-7细胞放射敏感性的实验研究  

Radiosensitization Of Celecoxib For Rectal HCA-7 Cells

作　　者：宫文静[1] 孙萍[1] 姚连昌[1] 刘爱娜[1] 由栋[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院肿瘤内二科,山东烟台264000

出　　处：《现代生物医学进展》2012年第5期868-871,864,共5页Progress in Modern Biomedicine

摘　　要：目的:观察环氧合酶-2(COX-2)抑制剂塞来昔布对直肠癌HCA-7细胞株的放射敏感性及探讨其机制。方法:采用MTT法检测塞来昔布作用不同时间对直肠癌HCA-7细胞株增殖的影响,计算出塞来昔布的半数抑制浓度IC50;HCA-7细胞克隆形成实验用于检测塞来昔布对HCA-7细胞的放射敏感性,并绘制存活曲线;流式细胞仪(FCM)测定塞来昔布对HCA-7的细胞周期的影响。结果:塞来昔布对HCA-7细胞株的抑制率随时间的延长而升高,48h的IC50是40.19μmol/L;照射组+药物组的SF2、D0、Dq、SER较单纯照射组均有所下降。塞来昔布使HCA-7细胞发生G2和M期阻滞,并抑制S期的比例。结论:塞来昔布能增加直肠癌HCA-7细胞的放射敏感性。Objective： To study the radiosensitization of cyclooxygenase-2 （COX-2） inhibitor celecoxib on rectal HCA-7 cells in vitro and try to venture into possible radiosensitizing mechanisms. Methods： MTT assay was used to measure the proliferation inhibition of celecoxib on HCA-7 cells and determine the concentration IC50. Rectal HCA-7 exposed to diffrerent dose of radiation with or without the 20% of IC50 concentration of celecoxib. Celecoxib＇s radiosensitization was measured by clongenic assay,and survival curve was described. The cell cycle distribution was analyzed by flow cytometry （FCM）. Results： Inhibition of cell proliferation seemed more dependent on the time. The value of SF2,D0,Dq of the group of radiation combined with celecoxib was significantly lower than that of radiation alone group. Cell cycle analysis showed that the cell population with celecoxib in G2/M phase increased and in S phase decreased. Conclusion： Celecoxib could enhance the radiotherapy on HCA-7 cells by promotion of apoptosis, inhibition of sublethal damage repair and facilitation of redistribution of tumor cell cycle.

关 键 词：塞来昔布 直肠癌 放射增敏 

分 类 号：R735.37[医药卫生—肿瘤]