RhoA/ROCK2信号通路在宫颈上皮内瘤样病变及宫颈癌组织中的临床意义  

Expression and significance of RhoA and ROCK2 in cervical intraepithelial neoplasia and cervical cancer

作　　者：顾颖[1] 张晔[1] 

机构地区：[1]南京医科大学附属无锡市妇幼保健院妇产科,江苏无锡214002

出　　处：《南京医科大学学报（自然科学版）》2012年第3期386-390,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金面上项目(08NM-UM081)

摘　　要：目的:研究RhoA、ROCK2在宫颈上皮内瘤样病变(cervical intraepithelial neoplasia,CIN)及宫颈癌组织中的表达,探讨其与CIN疾病及宫颈癌进展的相关性。方法:应用免疫组化S-P法,检测10例宫颈炎、30例不同级别CIN、30例宫颈癌组织中RhoA、ROCK2蛋白的表达情况,分析比较其表达与临床病理特征的关系。结果:①RhoA、ROCK2蛋白在宫颈炎组织中的表达率均为0%,在CIN宫颈组织中的表达率分别为36.7%、30.0%,在宫颈癌组织中的表达率分别为83.3%、76.7%,两者在宫颈癌组织中的表达水平高于CIN及宫颈炎组织(P<0.01),从CIN进展为宫颈癌,二者的阳性表达率呈递增趋势。②RhoA、ROCK2蛋白表达与宫颈癌病理组织分级及淋巴结转移相关(P<0.05),与宫颈癌患者的年龄、临床分期无明显相关关系(P>0.05)。③RhoA、ROCK2蛋白二者表达水平呈正相关关系(P=0.005)。结论:RhoA、ROCK2蛋白的表达水平与CIN疾病及宫颈癌进展呈正相关,检测RhoA、ROCK对了解CIN及宫颈癌生物学行为和评估预后具有一定的临床价值。Objective：To investigate the expression of RhoA and ROCK2 in cervical intraepithelial neoplasia（CIN） and cervical cancer and its correlation with invasiveness.Methods： The expressions of RhoA and ROCK2 were examined respectively by immunohistochemical S-P method in 10 cases of cervicitis tissue,30 cases of cervical intraepithelial neoplasia and 30 cases of cervical cancer.The correlation between the immunohistochemical results and the clinicopathological features were analyzed.Results： ① The expression rate of RhoA and ROCK2 were 0% in cervicitis tissues,36.7% and 30.0% in cervical intraepithelial neoplasia,83.3% and 76.7% in cervical cancer,respectively.The positive rate was significantly higher in the cervical cancer group than in the normal control group（P 0.01）.From cervicitis tissue to cervical intraepithelial neoplasia and to cervical cancer,the positive expression rates of RhoA and ROCK2 were gradually increased.② RhoA and ROCK2 protein expression were correlated with histological grade and lymph node metastasis（P 0.05）,but not with age and clinical stage（P 0.05）.Conclusion： Expression of RhoA and ROCK2 may serve as a parameter for evaluating metastasisand prognosis in cervical cancer.

关 键 词：RHOA ROCK2 免疫组化 CIN 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]