鱼油对开放式腹部手术大鼠术后疲劳的影响及机制  

Effect of fish oil on postoperative fatigue of rats subject to open abdominal surgery： implications and mechanisms

作　　者：王强[1] 叶星照[1] 陈必成[2] 张培趁[1] 闫竞一[1] 余震[1] 

机构地区：[1]温州医学院附属第一医院普外科,325000 [2]温州医学院附属第一医院外科实验室,325000

出　　处：《中华实验外科杂志》2012年第4期622-624,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81070702）;浙江省重点扶植学科项目（07-F03）;浙江省医药卫生科技计划项目（2011ZDA018）

摘　　要：目的探讨鱼油对开放式腹部手术大鼠术后疲劳的影响及机制。方法将24只sD大鼠均分成对照组（C组）、手术组（S组）和鱼油干预组（F组）。术后观察行为学变化，检测白细胞介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—PX）含量；光镜观察脑海马组织形态，电镜观察其超微结构；高效液相检测中枢神经递质去甲肾上腺素（NE）、多巴胺（DA）、5-羟色胺（5-HT）。结果病理和电镜显示F组损伤较s组轻；术后高效液相分析表明中枢不同区域的神经递质含量不同。S组血清IL-1β、IL-6、TNF—α浓度分别为（60．02±11．08）、（50．13±4．45）、（41．08±2．17）ng／L，F组为（45．48±13．51）、（42．08±4．47）、（38．76±2．41）ng／L，均较C组增高（P〈0．01），F组较S组则明显降低（P〈0．01）；F组的血清SOD、GSH—PX含量较s组高（P〈0．01）；F组Morris水迷宫实验指标优于S组（P〈0．05）。结论鱼油能有效改善术后的炎症反应，减轻抗氧化防御系统的损伤，减轻中枢对外周手术创伤的应激，改善术后疲劳。Objective To investigate the effects of fish oil on postoperative fatigue （POF） and the action mechansims. Methods Twenty-four SD rats were randomly divided into control group （ group C, n = 8 ）, surgery group （ group S, n = 8 ） and fish oil treatment group （ group F, n = 8）. Postoperative behaviours were observed. Serum levels of interleukin （IL） -1-β, IL-6, tumor necrosis factor （TNF） -α, superoxide dismutase （SOD） and glutathione peroxidase （GSH-PX） were determined. Histological changes and ultra- structural changes of neurons in the hippocampus were examined with Hematoxylin and Eosin （HE） stai- ning, and under an electron microscope. The central levels of 5-hydroxytryptamine （5-HT）, dopamine （DA） and norepinephrine （NE） were measured by high-performance liquid chromatography-fluorescence. Results Histological and ultrastructural lesions in group F were milder than in group S. The contents of neurotransmitters were different. The levels of IL-1-β, IL-6 and TNF-α in group S [ （60. 02± 11.08 ）, （50. 13 ±4.45）, （41.08 ±2. 17） ng/L] and group F [ （45.48 ± 13.51）, （42. 08 ±4.47）, （38.76± 2. 41 ） ng/L] were higher than those in group C （P 〈 0.01）, and those in group F were lower than in group S （ P 〈 0. 01 ） ; The levels of SOD and GSH-PX in group F were higher than in group S （ P 〈 0. 01 ）. The results for Morris water maze in group F were superior to those in group S （ P 〈 0. 05 ）. Conclusion Fish oil could effectively ameliorate the postoperative inflammatory reaction, reduee damage to antioxidant defense system, alleviate the eentral stress, and improve POF.

关 键 词：开放式腹部手术 术后疲劳 鱼油 炎症 

分 类 号：R285.5[医药卫生—中药学]