血管内介入方法制作偏侧帕金森病猴模型  

Establishment of hemiparkinsonism model of rhesus induced by 1-methyl-4-phenyl-1,2,3,6-tetrahy- dropyridine

作　　者：王举磊[1,2] 秦怀洲[1,2] 朱俊玲[2] 高国栋[2] 徐国政[1] 马廉亭[1] 

机构地区：[1]广州军区武汉总医院神经外科,武汉430070 [2]第四军医大学唐都脑科医院神经外科

出　　处：《中华实验外科杂志》2012年第4期756-757,共2页Chinese Journal of Experimental Surgery

摘　　要：目的探讨血管内介入方法制作偏侧帕金森病（PD）猴模型的稳定技术和方法，评价其有效性及实用性。方法4只健康老年恒河猴，经右股动脉插管至颈内动脉注射1-甲基4．苯基-1，2，3，6-四氢吡啶（MPTP），剂量为1．0—1．2mg／kg，药物浓度为0．2g／L。术后2周进行运动功能评分，行阿扑吗啡诱转试验，10周后采用免疫组织化学方法检测中脑多巴胺能神经元变化。结果4只恒河猴制作模型成功。免疫组织化学检测示注药侧中脑黑质酪氨酸羟化酶（TH）阳性细胞形态改变，数量减少，注药侧平均为（20．3±4．7）个，正常侧平均为（66．3±11．9）个（P〈0．05）。结论血管内介入方法制作偏侧帕金森病猴模型是一种安全、有效的方法，但对硬件及技术条件有一定要求。Objective To establish the hemiparkinsonism model of rhesus monkey by unilateral internal carotid artery （ICA） infusion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine （MPTP） and evaluate the effectiveness and practicality. Methods Four healthy rhesus monkeys aged from 7 to 9 received MPTP injection through ICA by means of endovascular operation from the femoral artery. The dose of MPTP was 1.0-1.2 mg/kg, and the drug level was 0. 2 mg/mL. Motor function scale was evaluated 2 weeks after surgery. Results All the rhesus monkeys developed PD successfully. The tyrosine hydroxylase （TH）-immunoreactive neurons in the mesencephalon of the injection side were deformed and decreased, displaying significant difference with that in control side （20. 3 ±4. 7 vs 66. 3 ± 11.9, P 〈 0. 05 ）. Conclusion The rhesus monkey model of hemiparkinsonism could be induced by unilateral ICA infusion of MPTP and endo- vascular operation was safe and effective.

关 键 词：帕金森病 恒河猴 血管内介入 

分 类 号：R742.5[医药卫生—神经病学与精神病学]