海洋低温酶酶解贻贝的动力学研究  

Kinetic model for the enzymolysis of mussel by marine microtherm alkaline protease

作　　者：陈晓刚[1] 陈忻[1] 林少佳[1] 苗晴[2] 

机构地区：[1]佛山科学技术学院化学与化工系,广东佛山528000 [2]佛山科学技术学院信息科学与数学系,广东佛山528000

出　　处：《计算机与应用化学》2012年第3期262-268,共7页Computers and Applied Chemistry

基　　金：“十一五”国家科技支撑计划课题(2008BAD94B08);广东省2009院省合作项目(2009B091300018)“广东特色贝类活性物质规模化开发利用的新技术研究与示范”;广东省2010院省合作项目(2010B090900027)“海洋动物肽粉生产关键技术的研究与示范”

摘　　要：用海洋低温碱性蛋白酶在温度30℃、pH为10.00条件下,对贻贝肉蛋白进行酶解。用相关的动力学模型推导公式与实验相结合,研究其酶解机制。结果表明:在反应过程中存在着酶失活现象,以及底物存在着促进反应进行和抑制酶活性的双重作用;得到的水解度动力学方程及水解速率方程,结果表明酶催化水解速率随水解进程呈指数递减;失活常数k_d=13.989 min^(-1);动力学模型计算值与实验值平均相对偏差为5.80%,此结果说明该动力学模型与实验测量结果有很高的吻合性。Enzymatic hydrolysis of mussel meat protein was studied by marine microtherm alkaline protease under the conditions of temperature 30 ℃ and pH 10.00. The enzymolysis mechanism was researched with the method of combining related dynamics model derivation formula with experiments. Results showed that： the phenomenon of enzymes inactivation existed during the reaction, and substrates existed double actions of promoting reaction and restraining enzymes activity. The dynamics equation of hydrolysis degree and hydrolysis rate equation indicated that enzyme hydrolysis speed decreased exponentially with hydrolysis process. Inactivation constant ka was 13.989 rain^-1. The average relative deviation of dynamics model calculation values with experimental values was 5.80%, this result indicated that dynamic model and the experimental results had high coincidence.

关 键 词：贻贝 海洋低温碱性蛋白酶 酶解 动力学模型 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程] O643.1[轻工技术与工程—食品科学与工程]