Msx2和topo Ⅱ-α在鼻内翻性乳头状瘤中的表达及意义  

Expression and significance of Msx2 and topoⅡ-α in sinonasal inverted papilloma

作　　者：吴琼芳[1,2] 杨一兵[3] 吴锡芳[3] 赵川[4] 从林海[3] 阮标[3] 张俊铮[1] 

机构地区：[1]昆明医学院,2008级硕士研究生昆明650032 [2]河北省邯郸市第三医院耳鼻咽喉科,河北邯郸056001 [3]昆明医学院第一附属医院耳鼻咽喉科 [4]昆明医学院第一附属医院病理科

出　　处：《临床耳鼻咽喉头颈外科杂志》2012年第8期343-346,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：云南省应用基础研究面上项目(No:2010ZC124)

摘　　要：目的:探讨肌节同源盒基因同系物2(Msx2)和人拓扑异构酶Ⅱ-α(topoⅡ-α)在鼻内翻性乳头状瘤(SNIP)组织中的表达和意义,及其在SNIP恶变发生、发展过程中的关系。方法:采用免疫组织化学SP法分别检测Msx2和topoⅡ-α在32例SNIP,30例鼻息肉,30例恶变SNIP组织中的表达情况,其中SNIP组又根据病理形态分为轻度不典型增生组、中度不典型增生组和重度不典型增生组。结果:SNIP、恶变SNIP组织中Msx2的平均光密度值分别为0.2183±0.0598和0.2521±0.0761,均显著高于鼻息肉组织的0.1878±0.0372(P<0.05或0.01)。SNIP、恶变SNIP组织中topoⅡ-α的平均光密度分别为0.230 3±0.039 7和0.266 6±0.048 3,均显著高于鼻息肉组织的0.197 8±0.038 8(P<0.01)。Msx2和topoⅡ-α阳性表达在SNIP的3个病理形态分组间两两比较差异均有统计学意义(P<0.01或0.05),且在SNIP中的表达呈正相关(P<0.05)。结论:Msx2和topoⅡ-α在SNIP恶变的发生、发展中起重要作用,有可能成为SNIP基因治疗的靶点。Objective：To investigate the expression and significance of muscle segment homeobox2（Msx2） and topoⅡ-α in sinonasal inverted papilloma（SNIP）,and the relationship in the process of malignant transformation of SNIP.Method：Immunohistochemical method was used to detect the expression of Msx2 and topoⅡ-α in 32 cases of SNIP,30 cases of inflammatory nasal polyp（INP） and 30 cases of SNIP with carcinoma.According to the pathology results,SNIP were divided into mild atypical hyperplasia,moderate atypical hyperplasia and severe atypical hyperplasia.Result：The mean optical density of Msx2 in SNIP and SNIP with carcinoma tissues were 0.218 3±0.059 8 and 0.252 1±0.076 1,which were significantly higher than 0.187 8±0.037 2 in the INP tissue（P0.05 or 0.01）.The mean optical density of topoⅡ-α in SNIP and SNIP with carcinoma tissues were 0.230 3±0.039 7 and 0.266 6±0.048 3,which were significantly higher than 0.197 8±0.038 8 in the NIP tissue（P0.01）.There were significant difference of Msx2 and topoⅡ-α in SNIP between any two of the three groups divided according to pathological morphology（P0.01 or 0.05）.The expression of Msx2 and topoⅡ-α in SNIP were positively correlated（P0.05）.Conclusion：Msx2 and topoⅡ-α may play an important role in the occurrence and development of SNIP.So it can be used as new therapeutic targets.

关 键 词：鼻肿瘤 肌节同源盒基因同系物2 人拓扑异构酶Ⅱ-α 免疫组织化学 

分 类 号：R739.62[医药卫生—肿瘤]