黑素瘤抑制蛋白在转基因小鼠胚胎乳腺组织中的表达  

Expression of melanoma inhibitory protein in mammary buds of transgenic mice

作　　者：谢渭芬[1] 高勇 贺祥 李舰[1] 朱樑[1] 陈伟忠[1] 林勇[1] 

机构地区：[1]第二军医大学长征医院消化内科,上海200003 [2]长征医院肿瘤科 [3]第二军医大学科研部

出　　处：《第二军医大学学报》2000年第1期29-30,I002,共3页Academic Journal of Second Military Medical University

基　　金：国家"863"计划资助!项目(102-10-04-03)

摘　　要：目的：检测黑素瘤抑制蛋白（ＭＩＡ／ＣＤ－ＲＡＰ）在小鼠胚胎乳腺组织中的表达。方法：剖腹分离出１０．５～１６．５ ｄ 的ＭＩＡ／ＣＤ－ＲＡＰ启动子－半乳糖苷酶（２．２ｌａｃＺ）转基因小鼠胚胎，利用Ｘ－ｇａｌ全胚胎染色观察转基因的组织和细胞染色，并以免疫组织化学染色检测内源性ＭＩＡ／ＣＤ－ＲＡＰ蛋白在胚胎乳腺组织中的表达。结果：ＭＩＡ／ＣＤ－ＲＡＰ在孕期１１．５ ｄ 的小鼠胚胎乳腺上皮细胞中开始表达，至１３．５ ｄ 表达明显增强，而后逐渐减弱，至１６．５ ｄ 消失。结论：ＭＩＡ／ＣＤ－ＲＡＰ可能在乳腺的早期发育和乳腺癌发生中起重要作用。Objective: To investigate the expression of melanoma inhibitory protein (MIA) in mammary buds of transgenic mice. Methods: Transgenic embryos were collected from day 10.5 to 16.5 by Cesarean section. The transgene expression was detected by whole mount staining with X gal. Immunohistochemistry was carried out to detect endogenous MIA/CD RAP expression in mammary buds. Results: MIA/CD RAP expression in mammary epithelial cells began on day 11.5 p.c. and continued throughout day 15.5 p.c., with the strongest expression observed on day 13.5 p.c. Conclusion: MIA/CD RAP may play an important role in the early organogenesis of mammary gland and carcinogenesis of breast cancer.

关 键 词：黑素瘤抑制蛋白 小鼠 转基因 乳腺癌 

分 类 号：R737.902[医药卫生—肿瘤] Q78[医药卫生—临床医学]