瞬时受体电位M7通道蛋白对大鼠肝星状细胞凋亡的影响  

The effects of transient receptor potential melastaln 7 channel protein on apoptosis of rat hepatic stellate cells

作　　者：刘红[1] 李俊[1] 黄成[1] 黄艳[1] 周德喜[1] 

机构地区：[1]安徽省天然药物活性研究重点实验室安徽医科大学药学院,安徽合肥230032

出　　处：《中国药理学通报》2012年第4期486-489,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81072686;30873081;81102493);教育部博士学科点新教师基金项目(No20103420120001)

摘　　要：目的通过转染特异性siRNA,下调瞬时受体电位M7通道蛋白(TRPM7)在大鼠肝星状细胞系HSC-T6中的表达,研究其对HSC-T6凋亡的影响及内在机制。方法利用Li-pofectamineTM2000将特异性TRPM7-siRNA转染到HSC-T6内,分别运用流式细胞术检测HSCs凋亡变化;Western blot检测TRPM7、Caspase-3蛋白的表达;RT-PCR检测TRPM7,Caspase-3、Bid mRNA的表达。结果 TRPM7-siRNA明显抑制TRPM7 mRNA和蛋白的表达。与正常组或对照组相比,转染组细胞凋亡率、凋亡蛋白Caspase-3及凋亡基因Bid的表达均明显上调。结论特异性TRPM7-siRNA能明显抑制TRPM7表达,诱导HSCs凋亡,其机制可能与其诱导凋亡基因Bid表达上调有关。Aim To investigate the effects of downexpression of transient receptor potential melastatin 7（TRPM7） and its internal mechanisms on apoptosis through transfecting specific siRNA in rat hepatic stellate cell lines HSC-T6.Methods Specific TRPM7-siRNA was transfected by LipofectamineTM 2000.Cell apoptosis was measured by Flow Cytometry.TRPM7 and Caspase-3 protein expression were analyzed by Western blot;TRPM7,Caspase-3 and Bid mRNA expression were evaluated by RT-PCR.Results Suppression TRPM7 expression by siRNA significantly increased the apoptosis of HSCs,the expression of Caspase-3 protein and Bid mRNA compared with normal or control.Conclusions Specific TRPM7-siRNA not only inhibits the expression of TRPM7 but also induces apoptosis of HSCs.The enhanced gene expression of Bid may contribute to HSCs apoptosis.

关 键 词：瞬时受体电位M7通道 肝星状细胞 细胞凋亡 SIRNA CASPASE-3 BID 

分 类 号：R-332[医药卫生] R322.47