小檗胺对视网膜母细胞瘤HXO-RB_(44)细胞增殖的抑制作用  

Inhibitory Effect of Berbamine on Proliferation of Retinoblastoma HXO-RB_(44) Cells

作　　者：冯亦颖[1] 王巨存[2] 潘振华[3] 李正翔[4] 李燕[2] 

机构地区：[1]天津市南开医院眼科,300100 [2]天津市天津医院 [3]天津医科大学总医院肺癌研究所 [4]天津医科大学总医院

出　　处：《天津医药》2012年第4期356-358,共3页Tianjin Medical Journal

摘　　要：目的:探讨小檗胺对视网膜母细胞瘤HXO-RB44细胞增殖和细胞周期的影响。方法:体外培养视网膜母细胞瘤HXO-RB44细胞,四甲基偶氮唑蓝(MTT)法测定0、2、4、8、16、32mg/L小檗胺作用24、48和72h后对HXO-RB44细胞增殖的抑制率,流式细胞仪检测0、4、8、16mg/L小檗胺作用HXO-RB44细胞24h后的细胞周期。结果:随着小檗胺浓度的增加和作用时间的延长,其对HXO-RB44抑制率逐渐增加,作用24、48、72h的IC50分别为25.26、10.94和6.25mg/L。流式细胞分析显示,与空白对照组比较,小檗胺可显著抑制细胞周期,使G2/M期细胞增多(P<0.01)。结论:小檗胺在体外可抑制视网膜母细胞瘤HXO-RB44细胞的增殖,并可诱导G2/M期阻滞,可能成为一种有效的抗视网膜母细胞瘤药物。Objective： To investigate the effect of berbamine （BBM） on the proliferation and cell cycle of retinoblastoma HXO-RB44 cells. Methods： HXO-RB44 ceils were cultured in vitro. The inhibitory effect of BBM on the cell Foliferation was assayed by MT＇F method after being interfered for 24 h, 48 h and 72 h by 0, 2, 4, 8, 16 and 32 mg/L, respectively. The cell cy- cles were analyzed by flow cytometry （FCM） after being interfered 24 h by 0, 4, 8 and 16 mg/L BBM, respectively. Results： The cell proliferation was significantly inhibited by BBM in a dose-dependent manner （P 〈 0.05）. Values of IC50 were 25.26, 10.94 and 6.25 mg/L for 24 h, 48 h and 72 h after treatment, respectively. The result of FCM analysis showed that BBM significantly inhibited the cell cycle, and increased the number of ceils in G2/M phase compared with those of control group （P 〈 0.01）. Conclusion： Berbamine can inhibit the proliferation of HXO-RB44 cells and induce cell cycle arrest in G2/M phase, which suggests that BBM may be an effective anti- retinoblastoma drug. g

关 键 词：小檗胺 视网膜母细胞瘤 细胞增殖 细胞周期 体外研究 剂量效应关系 药物 

分 类 号：R739.7[医药卫生—肿瘤]