ID1基因在急性髓系白血病中的表达及其临床意义  

Expression and clinical significance of ID1 gene in acute myeloid leukemia

作　　者：陆滢[1] 汪琼[2] 牧启田[1] 陈志妹[3] 楼基余[3] 倪万茂[3] 金洁[3] 

机构地区：[1]浙江省宁波市第一医院血液科,315000 [2]浙江省宁波市宁波大学医学院 [3]浙江大学附属第一医院、浙江大学血液病研究所

出　　处：《中华血液学杂志》2012年第4期278-281,共4页Chinese Journal of Hematology

基　　金：国家高技术研究发展计划（863计划）（2006AA02A405）

摘　　要：目的探讨分化抑制因子1（ID在）基因在急性髓系白血病（AML）患者中的表达及其临床意义。方法采用实时定量PCR（RQ．PCR）方法检测114例初发成人AML患者ID在基因的表达，并分析其临床意义。结果114例AML患者均检测到ID在基因表达，中位表达水平8525（57—11233238）；AML不同核型预后组之间ID在基因表达水平有明显差异，预后不良组明显高于预后中等组[中位表达水平分别为36840（336—11233238）和6630（66—1840798）]（P=0．006）；ID在基因表达水平与年龄[t〉60岁患者明显高于〈60岁患者（P=0．002）]和WBC[≥10×10^9／L患者明显高于〈10×10^9／L患者（P=0．005）]相关；在年龄〈60岁AML患者中，第1个疗程结束后未获得完全缓解（CR）患者伴有ID在基因高表达（CR与未CR患者中位表达水平分别为1268和9537，P=0．010）。结论ID在基因高表达多见于AML核型预后不良和高龄患者，与AML不良预后相关；ID在基因可能是成人AML患者不良预后的分子标志。Objective To explore the expression and clinical significance of ID1 gene in acute myeloid leukemia （AML） patients. Method Real-time quantitative PCR（RQ-PCR） was used to test the expression level of ID1 gene in 114 de novo adult AML patients, and the clinical features of these patients were analyzed. Results ID1 gene transcript levels were detectable in BM mononuelear cells from 114 patients with AML, the median expression level of all samples was 8525 （range ：57 - 11 233 238）. There was a statistically significant difference on expression level of ID1 gene among the three different cytogenetic prognosis groups, and the poor prognosis group （ median ： 36 840, range ： 336 - 11 233 238 ） harbored the significantly higher level of ID1 gene than the intermediate prognosis group （Median ：6630, range：66 -1 840 798 ）（ P = 0. 006）. The expression level of ID1 gene was positively associated with older age （age 360 years vs 〈 60 years, P =0.002） and higher WBC count （WBC 310 ×10^9/L vs 〈 10 ×10^9/L, P =0. 005）. Young patients （ age 〈 60 years） who were not obtained the complete remission （non-CR） after the first cycle of chemotherapy harbored the high level of ID1 gene （ Median： 9537 of non-CR vs 1268 of CR, P =0. 010）. Conclusions High expression level of ID1 gene was mostly seen in AML patients with adverse cytogenetics and older age （age 360 years）, and may be associated with poor prognosis of AML. ID1 gene might be a prognostic molecular marker of AML.

关 键 词：分化抑制因子 白血病 非淋巴细胞 急性 预后 

分 类 号：R733.71[医药卫生—肿瘤]