前列腺增生上皮细胞对间质细胞周期素I表达影响  

Epithelial cells suppress the cyclin I expression of stromal cells in benign prostatic hyperplasia

作　　者：杨小丽[1] 宣强[2] 莫曾南[2] 

机构地区：[1]广西医科大学医学科学实验中心,广西南宁530021 [2]广西医科大学第一附属医院泌尿科学研究所

出　　处：《中国公共卫生》2012年第4期484-485,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(39960074);广西青年科学基金(桂科青0339024)

摘　　要：目的观察前列腺增生上皮细胞对间质细胞细胞周期素I(cyclin I)基因表达的影响。方法分离良性前列腺增生(BPH)组织的间质细胞与上皮细胞,经细胞形态学及免疫组织化学方法证实后,构建上皮/间质细胞共培养模型;分别提取单独、共培养条件下间质细胞的mRNA,逆转录-聚合酶链反应(RT-PCR)检测cyclin I表达的变化。结果成功分离2种不同形态的细胞,并仅在上皮细胞中检测到前列腺特异性抗原(PSA)阳性表达,间质细胞中检测结蛋白(desmin)的阳性表达;共培养模型后,逆转录-聚合酶链反应(RT-PCR)检测发现,在有上皮细胞共培养的间质细胞中,cyclin I基因mRNA的表达低于在单独培养的间质细胞的表达;cyclin I/甘油醛3磷酸盐脱氢酶(G3PDH)的灰度比值分别为(1.001±0.29)和(1.117±0.31),差异有统计学意义(P<0.05)。结论前列腺增生上皮细胞可抑制间质细胞cyclin I基因的表达。Objective To investigate the effect of epithelial cells on the cyclin I expression of stromal cells in benign prostatic hyperplasia（BPH）.Methods The stromal-epithelial co-cultured model was constructed after prostate epithelial cells and stromal cells were separated and identified from the tissue of benign prostatic hyperplasia with cell morphology and immunohistochemistry.The expression of cyclin I mRNA in stromal cells with or without the epithelial cells co-cultured was detected with reverse transcription PCR（RT-PCR）.Results The prostate-specific antigen（PSA） was only detected in epithelial cell,and desmin was only detected in stromal cells.After the stromal-epithelial co-cultured model was constructed,the expression of cyclin I was detected.Cyclin I mRNA was lower in stromal cells with epithelial cells co-cultured than that in stromal cells without epithelial cells co-cultured.The ratio of gray level between cyclin I to glycerol-3-phosphate dehyclrogenase（G3PDH） in stromal cells with or without epithelial cells co-cultured was 1.001±0.29 and 1.117±0.31（P〈0.05）.Conclusion The epithelial cells can suppress cyclin I expression in stromal cells in BPH.

关 键 词：良性前列腺增生(BPH) 间质/上皮细胞共培养模型 CYCLIN I 

分 类 号：R697.535[医药卫生—泌尿科学]