缺氧时5-Fu、As_2O_3联合及序贯治疗对肝癌细胞凋亡的影响  

During Hypoxia 5-fluorouracil Arsenic Trioxide Combination and Sequential Treatment of Liver Cancer Cells Influence

作　　者：周婷[1] 刘铁夫[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,150001

出　　处：《实用癌症杂志》2012年第2期114-117,共4页The Practical Journal of Cancer

摘　　要：目的探讨缺氧时5-氟尿嘧啶(5-fluorouracil,5-Fu)和三氧化二砷(As2O3)联合及序贯治疗对肝癌细胞凋亡的影响。方法采用MTT法,分析2种药物联合治疗及序贯治疗对肝癌细胞BEL-7402的细胞毒作用,流式细胞仪检测细胞周期和凋亡情况。结果与单用5-Fu及序贯用药相比,联合用药毒性明显增强,与单用5-Fu相比序贯用药毒性明显增强。联合用药可增加G1期细胞,减少S期细胞,序贯用药可减少G1期细胞,增加S期细胞,且联合用药组细胞凋亡率较高。结论 As2O3、5-Fu可导致细胞凋亡,两者联合有协同作用,与序贯用药相比,联合用药细胞毒作用更强,细胞凋亡率更高,治疗效果更强,实验结果可为临床提供参考依据。Objective To study the effect of combined or sequential use of 5-fluorouracil（5-Fluorouracil,5-Fu） and arsenic trioxide（As2O3） in the treatment of hepatocellular carcinoma.Methods MTT method was used to evaluate the toxicities to the human liver cell BEL-7402,and flow cytometry was used to evaluate the apoptosis of the cell after treating by combined or sequential use of 5-Fu and arsenic trioxide.Results Compared to sequential use of the two drugs,combined use of 5-Fu and arsenic trioxide significantly increased drug toxicity,increased G1 phase cell,decreased the s-phase cells,and increased the apoptosis of BEL-7402.Conclusion Combined use of As2O3 and 5-Fu can induced more apoptosis and is more effective in treating human liver cell BEL-7402.

关 键 词：缺氧 5-氟尿嘧啶 三氧化二砷 联合治疗 序贯治疗 

分 类 号：R735.7[医药卫生—肿瘤]