Effect of Endophytic Fungi AL12 on the Metabolites Distribution in Organs of Atraetylodes lancea  

内生真菌AL12对茅苍术代谢产物器官分配的影响(英文)

作　　者：高映雪[1] 李蕾[1] 戴传超[1] 

机构地区：[1]南京师范大学生命科学学院,江苏省微生物资源产业化工程技术研究中心,江苏省微生物与功能基因组学重点实验室,江苏南京210046

出　　处：《Agricultural Science & Technology》2012年第4期798-803,共6页农业科学与技术（英文版）

基　　金：Supported by the National Natural Science Foundation of China(31070443,30970523);the National Science Foundation for Talents Training in Basic Science,China(J1103507);the Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions,China~~

摘　　要：[Objective] To examine the effect of endophytic fungi ALl2 （Gilmaniella sp.） on metabolites distribution in organs of Atractylodes lancea. [Method] Endophytic fungi ALl2 was inoculated on Atraetylodes lancea plantlets in tissue culture, and the distribution of cellulose, hemicellulose, lignin, soluble sugar in leaves and roots of the inoculated group were detected. The weight of leaves and roots were compared. Gas Chromatography was used to analyze the volatile oil components. [Result] Compared with the control group, the average fresh weight and dry weight of leaves and roots of A. lancea which had been symbiosed with ALl2 increased significantly. The content of lignin and soluble sugar increased in the leaves of the inoculated group, and the content of cellulose, hemicellulose, lignin, soluble sugar and volatile oil also increased in roots. [Conclusion] The results indicate that symbiosed with ALl2 is benefit for the development of A. lancea roots and can promote the transfer and accumulation of the medicinal components to the roots.[目的]研究内生真菌孔球孢霉AL12号菌株对茅苍术组培苗生长及其代谢产物器官分配的影响。[方法]将内生真菌AL12与茅苍术组培苗进行共培养,比较感染前后茅苍术叶、根重量差异,检测接种AL12的组培苗中纤维素、半纤维素、木质素以及可溶性糖在叶片和根中的分布,并利用气相色谱分析挥发油组分。[结果]茅苍术与AL12共生后,与对照组相比,其组培苗叶片和根的鲜重和干重均有明显增加,且叶片木质素和可溶性糖含量增加,根部纤维素、半纤维素、木质素、可溶性糖及挥发油含量均有提高。[结论]该研究结果表明与AL12共生有利于茅苍术根部发育且能促使药用成分向根中转移、积累。

关 键 词：Atractylodes lancea Endophytic fungi METABOLITE Organ allocation 

分 类 号：S567.239[农业科学—中草药栽培]