ILK与HSP90相互作用对乳腺癌细胞迁移影响及其机制的探讨  

Influence of ILK-HSP90 interactions on cell migration in breast cancer cells and its mechanism

作　　者：孙晓杰[1] 林梁[2] 赵玫[2] 黄常志[2] 

机构地区：[1]齐齐哈尔医学院临床生物化学教研室,黑龙江齐齐哈尔161006 [2]中国医学科学院北京协和医学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室,北京100021

出　　处：《中华肿瘤防治杂志》2012年第3期161-165,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家重点基础研究发展计划(973)项目(2007CB914704);齐齐哈尔市科技局指导项目

摘　　要：目的:研究ILK与HSP90的相关性及其在肿瘤侵袭转移中的作用。方法:免疫共沉淀方法检测ILK与HSP90的相互作用;用反义ILK质粒转染乳腺癌细胞,建立稳定表达反义ILK的细胞系;蛋白质印迹法检测细胞内源性ILK的表达;细胞增殖实验和划痕实验观察细胞增殖和迁移情况;蛋白质印迹法检测E-cadherin、MMP-9以及β-catenin的表达。结果:在IEC18和MA891细胞中均检测到ILK与HSP90α和HSP90β的相互作用;反义ILK在抑制细胞内源性ILK表达的同时促进HSP90β的表达;与对照组细胞相比,反义ILK使MA891细胞生长速度减慢、运动迁移能力下降;其在增加E-cadherin表达的同时抑制MMP-9以及细胞核β-catenin的表达。结论:乳腺癌细胞中存在ILK与HSP90的相互作用,反义ILK通过抑制Wnt/β-catenin信号通路关键分子β-catenin的核积累而抑制乳腺癌细胞迁移。OBJECTIVE： To investigate the relationship between ILK and HSP90,and its role in tumor invasion and metastasis.METHODS： The interaction of ILK and HSP90 was confirmed by coimmunoprecipitation experiment.Stable MA891 cell line expressing antisense ILK proteins was generated by pCDNA3-anti-ILK plasmid transfection.Expression of endogenous ILK was detected by western blotting.Cell proliferation and migration was observed by MTT and wound healing assay.Expression of E-cadherin,MMP-9 and β-catenin was detected by Western Blotting.RESULTS： There are interaction between ILK and both HSP90α and HSP90β in IEC18 and MA891 cells.Antisense ILK inhibited the expression of endogenous ILK as well as promotes that of HSP90β in MA891 cells.Compared with the control cells,the growth of MA891 cells was suppressed and the ability of cell migration was descended after antisense ilk expression.Antisense ILK increasing the expression of cell adhesion molecule E-cadherin and decreasing that of tumor metastasis associated gene MMP-9 and nuclear β-catenin.CONCLUSIONS： There are interaction between ILK and HSP90 in breast cancer cells.Antisense ILK inhibits cell migration through suppressing the accumulation of nuclear β-catenin which is Wnt/β-catenin signal pathway key element.

关 键 词：乳腺肿瘤 ILK HSP90 细胞迁移 信号传导 印迹法 蛋白质 

分 类 号：R737.9[医药卫生—肿瘤]