高效液相色谱法测定苦荞中的β-谷甾醇  

Determination of β-sitosterol in tartary buckwheat by HPLC

作　　者：彭镰心[1] 王姝[2] 胡一冰[1] 邹亮[1] 邓丽君[1] 饶轩臣[1] 何鑫怡[1] 赵钢[1] 

机构地区：[1]成都大学生物产业学院,四川成都610106 [2]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《西南民族大学学报（自然科学版）》2012年第2期247-251,共5页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：国家星火计划(2010GA812002);四川省中管局项目(2010-78)

摘　　要：目的：建立HPLC法测定苦荞不同品种和不同部位β-谷甾醇含量.方法：色谱柱：Kromasil-ODS C18柱（250mm×4.6 mm,5μm）,流动相：100%甲醇,检测波长：205 nm,流速：1 ml/min,柱温：30℃,进样量：20μl.结果：β-谷甾醇在103~1854μg（r=0.9999）线性良好,平均回收率为100.59%,RSD=2.05%（n=6）,检测34个苦荞品种中β-谷甾醇含量范围为4.1～65.3 mg/100g,不同部位中苦荞麸皮β-谷甾醇含量最高,为116 mg/100 g,最低的为心粉.结论：该方法准确可靠、简便快速,可用于苦荞的质量评价.Objective： To establish the method to detect β-sitosterol in different cultivars and parts of tartary buckwheat by HPLC.Methods： The Kromasil-ODS C18（250 mm×4.6 mm,5 μm） is used,with liquid of methanol as mobile phase.The detection wavelength is 205 nm,the column temperature is 30 ℃,and the flow rate is 1.0ml/min.Results： β-sitosterol shows a good linear relationship within the range of 103~1854μg（r=0.9999）,the recovery rate is 100.59%,and RSD is 2.05%（n=6）.The β-sitosterol contents of tartary buckwheat in 34 cultivars are within the range of 4.1～65.3 mg/100g.The β-sitosterol contents of tartary buckwheat in different parts are compared,the highest is bran（116 mg/100 g）,the lowest is center flour.Conclusion： The method is accurate and reliable,simple and rapid,and can be used to evaluate the quality of tartary buckwheat.

关 键 词：高效液相色谱 苦荞 Β-谷甾醇 

分 类 号：R284[医药卫生—中药学]