双价基因抗虫棉及其抗虫性研究  

The Study of Insect-resistant Transgenic Cotton Habouring Double-gene and Its Insect-resistance

作　　者：李付广[1] 崔金杰[1] 刘传亮[1] 武芝霞[1] 李凤莲[1] 周勇[1] 李秀兰[1] 郭三堆[2] 崔洪志[2] 

机构地区：[1]中国农业科学院棉花研究所 [2]中国农业科学院生物技术研究所

出　　处：《中国农业科学》2000年第1期46-52,共7页Scientia Agricultura Sinica

基　　金：中华农业科教基金

摘　　要：采用花粉管通道法,将人工合成的GFMCryIA和修饰的CpT杀虫基因导入棉花优良品种(系)中。经生物鉴定,获得了抗虫植株。PCR分析表明,Bt和CpT基因均呈阳性反应。通过对转基因材料抗虫性研究,发现R1代植株中,不同转化体对棉铃虫的抗性存在着差异;双价抗虫材料ZGK9708R3代群体抗虫性较为稳定。By transformation method of the pollen tube pathway, the synthesized GFM CryIA gene and modified CpTⅠ gene were transformed into the elite cotton(Gossypium hirsutum L.) varieties(lines). After the field and lab identifications, the insect resistant transgenic plants were obtained. PCR detection results indicated the existence of the synthesized GFM CryIA gene and modified CpTⅠ gene respectively. In R 1 the bollworm resistances of each transformant appeared different, and the insect resistances of ZGK9708 R 3 generations performed stable.

关 键 词：双价抗虫基因 陆地棉 遗传转化 棉铃虫 抗虫性 

分 类 号：S562.03[农业科学—作物学]