人外周血单个核细胞CD14基因的扩增及序列测定  

Amplification and sequence of human CD14 gene from human mononuclear cell in peripheral blood

作　　者：张万江[1] 鲍朗[1] 邱洪宇[1] 晏菊芳[1] 胡昌华[1] 张会东[1] 

机构地区：[1]华西医科大学感染免疫研究室,成都610041

出　　处：《中华医学遗传学杂志》2000年第2期129-131,共3页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金!(39770663);四川省学术和技术带头人培养基金!(4989923)

摘　　要：目的　介绍一种获取人 CD14基因的简易方法。方法　利用 CD14基因组成的特点 ,采用聚合酶链反应技术以人外周血单个核细胞基因组 DNA为模板扩增获取人 CD14基因。结果　从人外周血单个核细胞基因组 DNA中成功扩增出大小约 1.1kb的人 CD14基因 ,并且经基因序列测定表明 ,克隆的人CD14基因序列与文献报道完全相同。结论　以人外周血单个核细胞基因组 DNA为模板 ,扩增人 CD14基因的方法是一种获取 CD14基因简捷、有效的方法。Objective To introduce a new method for getting human CD14 gene.Methods Based on the character of CD14 gene, the authors amplified the CD14 gene directly from genome DNA of human mononuclear cell in peripheral blood by PCR for the first time. Results A human CD14 gene about 1.1kb was successfully amplified from genome DNA of human mononuclear cell in peripheral blood, and the sequence analysis has indicated that the CD14 gene cloned in this way is the same as the sequence reported by other authors. Conclusion It is a simple and effective method to obtain human CD14 gene from genome DNA of human mononuclear cell in peripheral blood.

关 键 词：单个核细胞 CD14基因 序列测定 

分 类 号：R394.3[医药卫生—医学遗传学]