超临界流体萃取法测定六应丸中的冰片和丁香酚的含量  

Determination of Major Components in Liuyingwan Pills by Supercritical Fluid Extraction(SFE)

作　　者：陈斌[1] 陈伟[2] 方慧生[1] 杨根金[1] 吴玉田[1] 

机构地区：[1]第二军医大学药学院,上海200433 [2]中国人民解放军福州医学高等专科学校,福建福州350005

出　　处：《中国中药杂志》2000年第4期215-217,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目!(39770904)

摘　　要：目的 :建立用超临界流体萃取 (SFE)技术和毛细管气相色谱 (CGC)的联用技术测定中成药六应丸中主要成分冰片和丁香酚的含量的方法。方法 :采用正交设计法考察了影响超临界流体萃取因素的主效应及最佳萃取条件 ,并以毛细管气相色谱法进行定量分析。结果 :SFE的最佳萃取条件为 :压力 48 2MPa ,温度 6 0℃ ,改性剂量 0 2ml,静态萃取时间 10min。结论 :SFE CGC法测定六应丸中冰片和丁香酚快速、简便、准确。Objective: On the basis of SFE and CGC to establisha method for fhe qualitative and quantitative analysis of major components in Liuyingwan pills Method: Orthogonal design and variance analysis were used to optimize the four variables (temperature, pressure, volume of modifier and static extraction time)of SFE,and the quantification was completed by CGC Result: The optimized conditions are as follows: pressure 48 2 MPa,temperature 60 ℃, 0 2 ml ethyl acetate as modifier and static extraction 10 min The recoveries of borneol and eugenol are 101 93%( RSD 1 56%)and 98 43%( RSD 2 26%)respectively Conclusion: Compared with conventional methods, SFE is rapid, convenient and accurate in determining borneol and eugenol

关 键 词：超临界流体萃取 冰片 丁香酚 六应丸 含量测定 

分 类 号：R284[医药卫生—中药学]